Schneider P E, Alonzo G, Nakamoto T, Falster A U, Simmons W B
Department of Pediatric Dentistry Louisiana State University Medical Center, New Orleans 70119, USA.
Caries Res. 1995;29(4):285-90. doi: 10.1159/000262083.
The purpose of this study was to evaluate the effects of dietary caffeine during gestation and lactation on the acid solubility of molar teeth of weanling rats. Nineteen pregnant dams were divided into two groups. The 9 dams in the control group were fed a 20% protein diet supplemented with caffeine (2 mg/100 g BW) throughout the experiment. At birth, 8 pups were randomly assigned to each dam. Pups were killed on day 22. The 1st and 2nd molars were removed from each pup's maxilla and mandible. Four randomly selected molars from each litter were placed in a chamber and bathed with a flow of acid solution and the amount of mineral dissolved from the enamel was determined. The results showed that the amount of dissolved Ca and Mg from enamel surfaces of 1st molars from rats in the caffeine group after exposure to acid was consistently greater than that of the non caffeine group. In the 2nd molars there was no significant difference between caffeine and noncaffeine groups. Scanning electron microscopy revealed an alteration of the enamel surface of the 1st molars of the caffeine group after acid exposure. These results indicate that caffeine intake during gestation and lactation would have a deleterious effect on dental enamel of 1st molars in newborn rats.
本研究的目的是评估妊娠期和哺乳期摄入膳食咖啡因对断奶大鼠臼齿酸溶解性的影响。19只怀孕的母鼠被分为两组。对照组的9只母鼠在整个实验过程中喂食含咖啡因（2毫克/100克体重）的20%蛋白质饮食。出生时，每只母鼠随机分配8只幼崽。幼崽在第22天被处死。从每只幼崽的上颌骨和下颌骨中取出第一和第二磨牙。从每窝中随机选取四颗磨牙放入一个腔室中，用酸性溶液冲洗，测定从牙釉质中溶解的矿物质含量。结果表明，咖啡因组大鼠第一磨牙牙釉质表面暴露于酸后溶解的钙和镁量始终高于非咖啡因组。在第二磨牙中，咖啡因组和非咖啡因组之间没有显著差异。扫描电子显微镜显示，咖啡因组第一磨牙在酸暴露后牙釉质表面发生了改变。这些结果表明，妊娠期和哺乳期摄入咖啡因会对新生大鼠第一磨牙的牙釉质产生有害影响。
