Sasahara H, Cheuk S L, Wink C S, Hashimoto K, Rossowska M J, Nakamoto T
Department of Oral Diagnosis, Nihon University, School of Dentistry, Matsudo, Japan.
Toxicol Lett. 1994 Jul;73(1):55-64. doi: 10.1016/0378-4274(94)90188-0.
The effects of caffeine intake in early life on bone structure later in life were studied in rats. At day 9 of gestation, dams were divided into 2 groups. Group 1 (control) received a 20% protein diet; group 2 received the 20% protein diet supplemented with caffeine (2 mg/100 g body weight). After birth pups were continuously fed their respective diets until day 93, when the diet of group 2 was replaced with a noncaffeine 20% protein diet. On day 388 animals from both groups were weighed, killed, and femora and mandibles were removed. Calcium, phosphorus, magnesium, zinc, hydroxyproline, and hexosamine concentrations were measured. Radiographs of some femora were taken and paraffin cross sections were made at the midshaft of others. Femora in the caffeine group were wider, periosteal bone area/total bone area was greater, the cross sectional area of femoral bone was smaller, and there were fewer osteocytes/bone area than in controls. Calcium, phosphorus, zinc, and hydroxyproline concentrations in the caffeine group were less in both bones of the caffeine group. These results indicate that if animals are exposed to caffeine during the rapidly growing period, changes occur in femoral bone which are similar to those that occur with aging.
研究了早期摄入咖啡因对大鼠后期骨骼结构的影响。在妊娠第9天，将母鼠分为2组。第1组（对照组）给予20%蛋白质饮食；第2组给予补充咖啡因（2毫克/100克体重）的20%蛋白质饮食。出生后，幼崽持续喂食各自的饮食直至第93天，此时第2组的饮食被换成不含咖啡因的20%蛋白质饮食。在第388天，对两组动物进行称重、处死，并取出股骨和下颌骨。测量钙、磷、镁、锌、羟脯氨酸和己糖胺的浓度。对一些股骨进行X线摄影，并对另一些股骨的中轴制作石蜡切片。咖啡因组的股骨更宽，骨膜骨面积/总骨面积更大，股骨横截面面积更小，且每单位骨面积的骨细胞数量比对照组少。咖啡因组两根骨头中的钙、磷、锌和羟脯氨酸浓度均较低。这些结果表明，如果动物在快速生长期间接触咖啡因，股骨会发生与衰老时相似的变化。
