Functional Food Center, Korea Institute of Science and Technology, Gangneung Institute, Gangneung, Gangwon-do 210-340, Republic of Korea.
Food Chem. 2013 Jun 15;138(4):2399-406. doi: 10.1016/j.foodchem.2012.11.057. Epub 2012 Nov 20.
In this study, a hydrophilic interaction chromatography (HILIC) condition was developed for the simultaneous determination of five major phlorotannins from an extract of Eisenia bicyclis (Kjellman) Setchell with good linearity (r(2)>0.999). Based on this method, the seasonal variations and extraction characteristics, in terms of total extraction yield and the content of the phlorotannins, were investigated under various extraction conditions. In results, the yields and phlorotannins were increased two-to-four times in summer (June-October) and then, were decreased to normal levels in winter (November-March). In the extraction of E. bicyclis, ethanol percentage in water, extraction time and washing time significantly affected the yield of the extract and the phlorotannins, whereas the temperature and the sample/solvent ratio impacted the extraction to a lesser degree. These results will be useful information in the application of this macroalga in the commercial areas related to nutraceuticals, pharmaceuticals, and cosmeceuticals.
在这项研究中，开发了一种亲水作用色谱（HILIC）条件，用于同时测定来自双环鳞木叶藻（Kjellman）Setchell 提取物中的五种主要岩藻多酚，具有良好的线性（r²>0.999）。基于该方法，在各种提取条件下，研究了总提取产率和岩藻多酚含量的季节性变化和提取特性。结果表明，在夏季（6 月至 10 月），产率和岩藻多酚增加了两到四倍，然后在冬季（11 月至 3 月）降至正常水平。在双环鳞木叶藻的提取中，水的乙醇百分比、提取时间和洗涤时间对提取物和岩藻多酚的产率有显著影响，而温度和样品/溶剂比对提取的影响较小。这些结果将为该大型藻类在与营养保健品、药品和化妆品相关的商业领域的应用提供有用信息。
