Institute of Traditional Medicine, National Yang-Ming University, Taipei 11221, Taiwan.
Faculty of Pharmacy, School of Pharmaceutical Sciences, National Yang-Ming University, Taipei 11221, Taiwan.
Molecules. 2020 Nov 8;25(21):5198. doi: 10.3390/molecules25215198.
is a well-known species for flavoring many food products and for formulation of perfume and medicated balm. It is also used to treat indigestion, stomach ulcers, liver disorders, and, as emmenagogue, diaphoretic, diuretic, nervine, and aphrodisiac. We examined antioxidant properties and bioactive compounds in various solvent extracts from the seeds of . Methanol, ethanol, and acetone extracts exhibited relatively strong antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide radical, and hydroxyl radical scavenging tests. Furthermore, methanol extracts also displayed significant anti-α-glucosidase activity. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated the ten higher content compounds and analyzed antioxidant and anti-α-glucosidase activities. Among the isolates, dehydrodiisoeugenol, malabaricone B and malabaricone C were main antioxidant components in seeds of . Malabaricone C exhibited stronger antioxidant capacities than others based on lower half inhibitory concentration (IC) values in DPPH and ABTS radical scavenging assays, and it also showed significant inhibition of α-glucosidase. These results shown that methanol was found to be the most efficient solvent for extracting the active components from the seeds of , and this material is a potential good source of natural antioxidant and α-glucosidase inhibitor.
是一种著名的香料，用于调味许多食品产品，并用于配制香水和药用香膏。它还用于治疗消化不良、胃溃疡、肝脏疾病，并且作为通经药、发汗药、利尿剂、滋补药和壮阳药。我们研究了不同溶剂从种子中提取的抗氧化特性和生物活性化合物。甲醇、乙醇和丙酮提取物通过 2,2-二苯基-1-(2,4,6-三硝基苯基)肼(DPPH)、2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)、超氧自由基和羟自由基清除试验显示出相对较强的抗氧化活性。此外，甲醇提取物还显示出显著的抗α-葡萄糖苷酶活性。通过 HPLC 分析对各种溶剂提取物的化学成分进行了检查和比较，我们分离出了十种含量较高的化合物，并分析了它们的抗氧化和抗α-葡萄糖苷酶活性。在所分离的化合物中，去氢二异丁香酚、马拉巴酮 B 和马拉巴酮 C 是种子中的主要抗氧化成分。马拉巴酮 C 基于 DPPH 和 ABTS 自由基清除试验中的较低半抑制浓度 (IC) 值，表现出更强的抗氧化能力，并且对α-葡萄糖苷酶也有显著的抑制作用。这些结果表明，甲醇被发现是从种子中提取活性成分的最有效溶剂，并且这种材料是天然抗氧化剂和α-葡萄糖苷酶抑制剂的潜在良好来源。
