State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Biosens Bioelectron. 2013 Jul 15;45:213-8. doi: 10.1016/j.bios.2013.01.041. Epub 2013 Feb 9.
We reported a reagent-free tubular biofilm reactor (BFR) based analytical system for rapid online biochemical oxygen demand (BOD) determination. The BFR was cultivated using microbial seeds from activated sludge. It only needs tap water to operate and does not require any chemical reagent. The analytical performance of this reagent-free BFR system was found to be equal to or better than the BFR system operated using phosphate buffer saline (PBS) and high purity deionized water. The system can readily achieve a limit of detection of 0.25 mg O2 L(-1), possessing superior reproducibility, and long-term operational and storage stability. More importantly, we confirmed for the first time that the BFR system is capable of tolerating common toxicants found in wastewaters, such as 3,5-dichlorophenol and Zn(II), Cr(VI), Cd(II), Cu(II), Pb(II), Mn(II) and Ni(II), enabling the method to be applied to a wide range of wastewaters. The sloughing and clogging are the important attributes affecting the operational stability, hence, the reliability of most online wastewater monitoring systems, which can be effectively avoided, benefiting from the tubular geometry of the reactor and high flow rate conditions. These advantages, coupled with simplicity in device, convenience in operation and minimal maintenance, make such a reagent-free BFR analytical system promising for practical BOD online determination.
我们报道了一种无试剂管状生物膜反应器（BFR）分析系统，用于快速在线生化需氧量（BOD）测定。BFR 使用来自活性污泥的微生物种子进行培养。它仅需要自来水即可运行，不需要任何化学试剂。研究发现，这种无试剂 BFR 系统的分析性能与使用磷酸盐缓冲盐水（PBS）和高纯度去离子水运行的 BFR 系统相当或更好。该系统可以很容易地达到 0.25mgO2 L(-1) 的检测限，具有优越的重现性、长期运行和储存稳定性。更重要的是，我们首次证实 BFR 系统能够耐受废水中常见的有毒物质，如 3,5-二氯苯酚和 Zn(II)、Cr(VI)、Cd(II)、Cu(II)、Pb(II)、Mn(II)和 Ni(II)，使得该方法能够应用于广泛的废水。生物膜脱落和堵塞是影响运行稳定性的重要因素，因此，大多数在线废水监测系统的可靠性可以有效避免，这得益于反应器的管状几何形状和高流速条件。这些优点，加上设备简单、操作方便和维护量小，使得这种无试剂 BFR 分析系统有望用于实际的 BOD 在线测定。
