School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, 266033, China.
Laboratory for Marine Geology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China.
Archaea. 2020 Oct 26;2020:8894925. doi: 10.1155/2020/8894925. eCollection 2020.
In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms, which could increase the content of microorganisms and prolong the service life of the biological element. The conductivity (COND) probe is used as a sensing element, so that the testing value can be read every second. In the program, the moving average method is used to process the collected data so that the value can be read every minute. National standard samples were detected to test the accuracy and stability of the method. The results showed that relative error and analytical standard deviations were less than 5%. Different polluted water was tested to evaluate its application range. The results showed that relative error was less than 5%. The results of the method are consistent with the results of the wastewater sample obtained by the BOD standard method. The proposed rapid BOD current sensing biosensor method should be promising in practical application of wastewater monitoring.
为了提高生化需氧量（BOD）快速测定方法的实用性，开发了一种基于电流与溶解氧（DO）相关性的高灵敏度快速 BOD 检测方法。在本实验中，采用作为测试微生物，并采用包埋法实现微生物的定量固定，从而提高微生物的含量，延长生物元件的使用寿命。采用电导率（COND）探头作为传感元件，可实现每秒读取一次测试值。在程序中，采用移动平均法处理采集的数据，可实现每分钟读取一次值。采用国家标准样品检测方法的准确性和稳定性。结果表明，相对误差和分析标准偏差均小于 5%。不同污染水进行测试，以评估其应用范围。结果表明，相对误差均小于 5%。该方法的结果与采用 BOD 标准方法获得的废水样品的结果一致。因此，提出的快速 BOD 电流传感生物传感器方法在实际废水监测中具有广阔的应用前景。
