Department of Food Science, University of Copenhagen, Frederiksberg, Denmark.
Appl Environ Microbiol. 2013 May;79(10):3311-4. doi: 10.1128/AEM.00012-13. Epub 2013 Mar 15.
Two phages, P793 and ΦLN04, sharing 80.1% nucleotide sequence identity but having different strains of Leuconostoc pseudomesenteroides as hosts, were selected for identification of the host determinant gene. Construction of chimeric phages leading to the expected switch in host range identified the host determinant genes as ORF21P793/ORF23ΦLN04. The genes are located in the tail structural module and have low sequence similarity at the distal end.
两种噬菌体 P793 和 ΦLN04 被选中进行鉴定,它们的宿主分别为肠膜明串珠菌不同菌株,具有 80.1%的核苷酸序列同一性。构建嵌合噬菌体导致预期的宿主范围转换,确定了宿主决定基因 ORF21P793/ORF23ΦLN04。这些基因位于尾部结构模块中,在远端具有低序列相似性。