Paul S, Sen A K, Banerjee N, Chatterjee A N, Das J
Biophysics Division, Indian Institute of Chemical Biology, Calcutta, India.
Biochem Biophys Res Commun. 1990 May 31;169(1):116-22. doi: 10.1016/0006-291x(90)91441-t.
Vibrio cholerae mutants resistant to common antibiotics and neutral and anionic detergents were isolated. Analysis of isolated outer membranes revealed a significant deficiency in the acylation of lipid A in the resistant strains. The content of amide-linked and ester-bound fatty acids in the lipid A of the mutant strains compared to that of the wild type was about 50-56% and 29-37% respectively. This defect was specific for lipid A as there was no change in the acylation of phospholipids. The reduction in fatty acid content of lipid A was reflected in the altered endotoxic properties in the mutant strains.
分离出了对常见抗生素以及中性和阴离子去污剂具有抗性的霍乱弧菌突变体。对分离出的外膜进行分析发现，抗性菌株中脂多糖A的酰化存在显著缺陷。与野生型相比，突变菌株脂多糖A中酰胺连接的脂肪酸和酯结合的脂肪酸含量分别约为50 - 56%和29 - 37%。这种缺陷是脂多糖A特有的，因为磷脂的酰化没有变化。脂多糖A脂肪酸含量的降低反映在突变菌株内毒素特性的改变上。
