Peng Xiaohua, Li Hong, Seidman Michael
Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St., Milwaukee, 53211, U.S.A.
European J Org Chem. 2010 Aug 1;2010(22):4194-4197. doi: 10.1002/ejoc.201000615. Epub 2010 Jun 17.
A highly efficient chemical ligation was developed for quantitative conjugation of PNA with DNA (PNA or peptide) using the copper-catalyzed azide-alkyne cycloaddition reaction. While PNAs with an alkyne at the C-terminus and an azide at the N-terminus have been used, an efficient click-click reaction occurs. The PNA click ligation is sequence-specific and capable of single nucleotide discrimination.
利用铜催化的叠氮化物-炔烃环加成反应，开发了一种高效的化学连接方法，用于PNA与DNA（PNA或肽）的定量共轭。虽然使用了在C端带有炔烃和在N端带有叠氮化物的PNA，但会发生高效的点击反应。PNA点击连接具有序列特异性，能够区分单核苷酸。
