蜂胶的化学成分、细胞毒性和抗氧化活性对 HCT-116 细胞系的影响。

Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line.

机构信息

Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.

出版信息

J Sci Food Agric. 2013 Sep;93(12):3001-9. doi: 10.1002/jsfa.6132. Epub 2013 Apr 18.

DOI:10.1002/jsfa.6132

PMID:23504630

Abstract

BACKGROUND

Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD.

RESULTS

The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 µg mg⁻¹), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC₅₀ values ranging from 26.33 to 143.09 µg mL⁻¹. Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity.

CONCLUSION

All tested propolis samples had pronounced cytotoxic and antioxidative activities.

摘要

背景

蜂胶是蜜蜂从各种植物的芽和渗出物中采集的一种复杂的树脂状粘性物质。由于其多功能的生物和药理活性，蜂胶广泛应用于医药、化妆品和食品中。本研究旨在评估不同浓度的蜂胶乙醇提取物（EEP）对人结肠癌细胞系 HCT-116 的细胞毒性和抗氧化作用，并将其与通过 HPLC-DAD 测定的组成进行比较。

结果

所有样品中含量最丰富的黄酮类化合物为白杨素、芹糖松属素和乔松素（12.697-40.811μgmg⁻¹），而主要的酚酸类物质为咖啡酸、阿魏酸和异阿魏酸。所有蜂胶样品对 HCT-116 细胞的生长均呈剂量和时间依赖性抑制，IC₅₀ 值范围为 26.33-143.09μgmL⁻¹。蜂胶样品细胞毒性活性的差异与其组成有关。所有 EEP 样品均能降低超氧阴离子自由基和亚硝酸盐水平，且具有较强的 DPPH 清除活性。