Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2-24-16, Naka-cho, Koganei, Tokyo, Japan.
Nanomedicine. 2013 Oct;9(7):855-63. doi: 10.1016/j.nano.2013.03.003. Epub 2013 Mar 16.
Femtoinjection has been proposed as a feasible approach for the targeted delivery of a decoy oligodeoxynucleotide (ODN) into a single ES cell for the study of transcription factor activity. Here, we evaluated the utility of decoy ODN delivery via femtoinjection in an ES cell model in which Venus fluorescent protein was expressed under the control of the tet-off system. Femtoinjection of a control decoy (Con-decoy) and a tetracycline response element decoy (TRE-decoy) into the cytoplasm had no apparent effect on Venus fluorescent protein expression; however, femtoinjection of the TRE-decoy into the nucleus successfully suppressed expression of the Venus fluorescent protein. We therefore conclude that it is feasible to suppress the activity of a transcription factor in a single ES cell by the delivery of a decoy ODN into the nucleus using the femtoinjection technique.
The authors of this novel basic science study successfully demonstrate a femtoinjection technique to deliver a decoy oligodeoxynucleotide into a single ES cell.
已经提出飞秒注射作为一种可行的方法，将一种诱饵寡脱氧核苷酸（ODN）靶向递送至单个胚胎干细胞中，用于研究转录因子活性。在这里，我们评估了通过飞秒注射将诱饵 ODN 递送至 Venus 荧光蛋白在 tet-off 系统控制下表达的胚胎干细胞模型中的效用。飞秒注射对照诱饵（Con-decoy）和四环素反应元件诱饵（TRE-decoy）到细胞质中对 Venus 荧光蛋白表达没有明显影响；然而，将 TRE-decoy 注射到细胞核中成功抑制了 Venus 荧光蛋白的表达。因此，我们得出结论，使用飞秒注射技术将诱饵 ODN 递送至细胞核中，可以抑制单个胚胎干细胞中转录因子的活性。
这项新颖的基础科学研究的作者成功地展示了一种飞秒注射技术，可将诱饵寡脱氧核苷酸递送至单个胚胎干细胞中。
