Hauser-Gerspach Irmgard, Mauth Corinna, Waltimo Tuomas, Meyer Jürg, Stübinger Stefan
Institute of Preventive Dentistry and Oral Microbiology, School of Dental Medicine, University of Basel, Hebelstrasse 3, 4056, Basel, Switzerland,
Lasers Med Sci. 2014 Jul;29(4):1329-37. doi: 10.1007/s10103-013-1303-8. Epub 2013 Mar 19.
This in vitro study examined (a) the anti-bacterial efficacy of a pulsed erbium-doped yttrium aluminum garnet (Er:YAG) laser applied to Streptococcus sanguinis or Porphyromonas gingivalis adhered to either polished or microstructured titanium implant surfaces, (b) the response of osteoblast-like cells and (c) adhesion of oral bacteria to titanium surfaces after laser irradiation. Thereto, (a) bacteria adhered to titanium disks were irradiated with a pulsed Er:YAG laser (λ = 2,940 nm) at two different power settings: a lower mode (12.74 J/cm(2) calculated energy density) and a higher mode (63.69 J/cm(2)). (b) After laser irradiation with both settings of sterile titanium, disks were seeded with 10(4) MG-63 cells/cm(2). Adhesion and proliferation were determined after 1, 4, and 24 h by fluorescence microscopy and scanning electron microscopy. (c) Bacterial adhesion was also studied on irradiated (test) and non-irradiated (control) surfaces. Adhered P. gingivalis were effectively killed, even at the lower laser setting, independent of the material's surface. S. sanguinis cells adhered were effectively killed only at the higher setting of 63.69 J/cm(2). Laser irradiation of titanium surfaces had no significant effects on (b) adhesion or proliferation of osteoblast-like MG-63 cells or (c) adhesion of both oral bacterial species in comparison to untreated surfaces. An effective decontamination of polished and rough titanium implant surfaces with a Er:YAG laser could only be achieved with a fluence of 63.69 J/cm(2). Even though this setting may lead to certain surface alterations, no significant adverse effect on subsequent colonization and proliferation of MG-63 cells or increased bacterial adhesion was found in comparison to untreated control surfaces.
(a) 脉冲掺铒钇铝石榴石（Er:YAG）激光对附着于抛光或微结构钛种植体表面的血链球菌或牙龈卟啉单胞菌的抗菌效果；(b) 成骨样细胞的反应；以及 (c) 激光照射后口腔细菌对钛表面的黏附。为此，(a) 用脉冲 Er:YAG 激光（λ = 2940 nm）在两种不同功率设置下照射附着于钛盘的细菌：较低模式（计算能量密度为 12.74 J/cm²）和较高模式（63.69 J/cm²）。(b) 用两种设置的激光照射无菌钛盘后，每平方厘米接种 10⁴ 个 MG - 63 细胞。在 1、4 和 24 小时后通过荧光显微镜和扫描电子显微镜测定细胞黏附和增殖情况。(c) 还研究了照射表面（试验组）和未照射表面（对照组）上的细菌黏附情况。即使在较低激光设置下，附着的牙龈卟啉单胞菌也能被有效杀灭，且与材料表面无关。仅在 63.69 J/cm² 的较高设置下，附着的血链球菌细胞才能被有效杀灭。与未处理表面相比，钛表面的激光照射对 (b) 成骨样 MG - 63 细胞的黏附或增殖以及 (c) 两种口腔细菌的黏附均无显著影响。只有在能量密度为 63.69 J/cm² 时，用 Er:YAG 激光才能有效净化抛光和粗糙的钛种植体表面。尽管该设置可能会导致某些表面改变，但与未处理的对照表面相比，未发现对 MG - 63 细胞随后的定植和增殖有显著不利影响，也未发现细菌黏附增加。
