评价满天星（Sphaeranthus amaranthoides Burm.f.）的抗氧化和伤口愈合潜力
Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm.f.
机构信息
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, India.
出版信息
Biomed Res Int. 2013;2013:607109. doi: 10.1155/2013/607109. Epub 2013 Feb 3.
BACKGROUND
Sphaeranthus amaranthoides commonly known as sivakaranthai is used in folklore medicine for the treatment of skin diseases.
METHODS
The antioxidant activity of the extract and its fraction was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant capacity, and total phenolic content. The tested plant extracts showed variable degrees of antioxidant activity. In the present study, methanolic extract of the whole plant of S. amaranthoides and a flavonoid fraction obtained from column chromatography were studied for wound healing activity by incorporating the sample in simple ointment base. Wound healing activity was studied in excision wound model in rats, following which, wound contraction, period of epithelization, hydroxyproline content, and collagen levels in the scab were studied.
RESULTS
Methanolic extract showed the highest antioxidant effect (72.05%) and diethyl ether extract has the least (29.34%) compared to the standard (74.53%). Treatment of wound with ointment containing 5% (w/w) methanolic extract and 5% (w/w) flavonoid fraction exhibited better wound healing activity than positive control (silver sulfadiazine). Finally, histopathology studies conformed wound healing activity in Sphaeranthus amaranthoides. The methanolic extract and flavonoid fraction exhibited good wound healing activity probably due to the presence of phenolic and flavonoid constituents. The methanolic extract and flavonoid fraction significantly enhanced the rate of wound contraction and the period of epithelialization comparable to silver sulfadiazine.
背景
Sphaeranthus amaranthoides 俗称 sivakaranthai，民间医学用于治疗皮肤病。
方法
采用 2,2-二苯基-1-苦基肼基（DPPH）自由基清除活性、总抗氧化能力和总酚含量评价提取物及其馏分的抗氧化活性。测试的植物提取物表现出不同程度的抗氧化活性。在本研究中，研究了 Sphaeranthus amaranthoides 全草的甲醇提取物和从柱色谱获得的黄酮类馏分，通过将样品掺入简单的软膏基质中，研究其对伤口愈合的作用。通过切除伤口模型在大鼠中研究伤口愈合活性，然后研究伤口收缩、上皮化时间、羟脯氨酸含量和结痂中的胶原水平。
结果
与标准品（74.53%）相比，甲醇提取物显示出最高的抗氧化效果（72.05%），而二乙醚提取物的效果最低（29.34%）。含有 5%（w/w）甲醇提取物和 5%（w/w）黄酮类馏分的软膏处理伤口显示出比阳性对照（磺胺嘧啶银）更好的伤口愈合活性。最后，组织病理学研究证实了 Sphaeranthus amaranthoides 的伤口愈合活性。甲醇提取物和黄酮类馏分可能由于存在酚类和类黄酮成分而表现出良好的伤口愈合活性。甲醇提取物和黄酮类馏分显著提高了伤口收缩率和上皮化时间，与磺胺嘧啶银相当。
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