Bioorganic & Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS), Allahabad 211007, India.
Bioorganic & Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS), Allahabad 211007, India.
Biomed Pharmacother. 2017 Dec;96:86-97. doi: 10.1016/j.biopha.2017.09.125. Epub 2017 Sep 29.
Oxidative stress and inflammation contribute as a key factor for retarding the process of dermal wound healing. Trianthema portulcastrum Linn. (TP) leaves reported to possess antioxidant, antifungal, anti-inflammatory and antibacterial properties, which could make TP a promising wound healing agent. The current study was aimed to estimate the antioxidant potential of the fractionated hydroethanolic extract of TP leaves and evaluate wound healing activity by excision and incision wound models along with the assessment of possible underlying mechanism. Ethyl acetate, chloroform and n-butanol fractions of the hydroethanolic extract of TP leaves were examined for in vitro antioxidant ability by DPPH method. Strongest antioxidant activity bearing n-butanol fraction (nBuTP) was further analyzed quantitatively by High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD). Wound healing potential of nBUTP using excision and incision wound model was studied. Wistar albino rats were randomly divided into four groups, containing six animals in each group; group I served as control treated with simple ointment base, group II was standard group, treated with povidone-iodine ointment USP (5%), group III treated with nBuTP 5% w/w ointment, and group IV treated with nBuTP 10%w/w ointment. All the groups were topically applied their respective ointments, once daily, till the complete healing achieved. Wound healing was assessed by analyzing % wound closure, hydroxyproline content, epithelialization period, tensile strength, enzymatic antioxidative status and inflammatory markers. Total phenolic and flavonoid content of the extract was estimated to be 112.32±1.12 and 84.42±0.47mg/g, respectively. HPLC-DAD of nBuTP confirmed the presence of chlorogenic acid (20.74±0.03), protocatechuic acid (34.45±0.02mg/g), caffeic acid (4.31±0.03mg/g) and ferulic acid (1.43±0.01mg/g). 5% and 10%w/w nBuTP ointment significantly accelerated the wound healing process dose-dependently in both wound models, evidenced by the faster rate of wound contraction, epithelialization, increased hydroxyproline content, high tensile strength, increased antioxidant enzyme activity, decreased the level of inflammatory markers compared to the control group. Histopathological studies also revealed the dose-dependant amelioration of wound healing by re-epithelialization, collagenation and vascularization of wounded skin sample in nBuTP treated groups. These results implicate potential medicinal value of nBuTP to heal dermal wounds.
氧化应激和炎症是延缓皮肤伤口愈合过程的关键因素。蕺菜（TP）叶被报道具有抗氧化、抗真菌、抗炎和抗菌特性，这使其成为一种有前途的伤口愈合剂。本研究旨在评估 TP 叶水醇提取物的馏分的抗氧化潜力，并通过切除和切口伤口模型评估伤口愈合活性，同时评估可能的潜在机制。通过 DPPH 法检查 TP 叶水醇提取物的乙酸乙酯、氯仿和正丁醇馏分的体外抗氧化能力。具有最强抗氧化活性的正丁醇馏分（nBuTP）进一步通过高效液相色谱-二极管阵列检测器（HPLC-DAD）进行定量分析。使用切除和切口伤口模型研究 nBUTP 的伤口愈合潜力。Wistar 白化大鼠随机分为四组，每组 6 只动物；第 I 组作为对照组，用简单软膏基质处理，第 II 组为标准组，用聚维酮碘软膏 USP（5%）处理，第 III 组用 nBuTP 5%w/w 软膏处理，第 IV 组用 nBuTP 10%w/w 软膏处理。所有组均每天局部涂抹各自的软膏，直至完全愈合。通过分析 %伤口闭合、羟脯氨酸含量、上皮化期、拉伸强度、酶抗氧化状态和炎症标志物来评估伤口愈合。提取物的总酚和类黄酮含量估计分别为 112.32±1.12 和 84.42±0.47mg/g。nBuTP 的 HPLC-DAD 证实存在绿原酸（20.74±0.03）、原儿茶酸（34.45±0.02mg/g）、咖啡酸（4.31±0.03mg/g）和阿魏酸（1.43±0.01mg/g）。5%和 10%w/w nBuTP 软膏在两种伤口模型中均能显著地、剂量依赖性地加速伤口愈合过程，这表现在伤口收缩、上皮化、羟脯氨酸含量增加、拉伸强度增加、抗氧化酶活性增加、与对照组相比，炎症标志物水平降低。组织病理学研究还表明，nBuTP 处理组通过再上皮化、胶原化和受伤皮肤样本的血管化，使伤口愈合得到剂量依赖性改善。这些结果表明 nBuTP 具有治疗皮肤伤口的潜在药用价值。
