Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea.
J Nat Prod. 2013 Apr 26;76(4):615-20. doi: 10.1021/np300824h. Epub 2013 Mar 19.
Artemisia iwayomogi has been used as a folk medicine for treating various diseases including inflammatory and immune-related diseases. Scopoletin (1) and scopolin (2) were isolated from this species. Scopoletin (1) showed more potent peroxyl radical-scavenging capacity, reducing capacity, and cellular antioxidant capacity compared to scopolin (2). The inhibitory effect of 1 on the receptor activator of nuclear factor κB ligand-induced osteoclastic differentiation of RAW 264.7 macrophage cells was also more potent than that of 2. The production of general reactive oxygen species (ROS) and superoxide anions during differentiation of preosteoclastic RAW 264.7 cells into osteoclasts was attenuated by compounds 1 and 2. These findings indicate that the suppressive effects of 1 and 2 on the differentiation of preosteoclastic RAW 264.7 cells is partially due to their intracellular antioxidant capacity, as they can scavenge ROS and play an important signaling role in the differentiation process.
青蒿已被用作民间药物，用于治疗各种疾病，包括炎症和免疫相关疾病。从该物种中分离出东莨菪内酯（1）和东莨菪苷（2）。与东莨菪苷（2）相比，东莨菪内酯（1）具有更强的过氧自由基清除能力、还原能力和细胞抗氧化能力。化合物 1 对核因子κB 配体诱导的 RAW264.7 巨噬细胞破骨细胞分化的抑制作用也强于化合物 2。在成骨前体细胞 RAW264.7 细胞向破骨细胞分化过程中，1 和 2 可减少一般活性氧（ROS）和超氧阴离子的产生。这些发现表明，化合物 1 和 2 对成骨前体细胞 RAW264.7 细胞分化的抑制作用部分归因于它们的细胞内抗氧化能力，因为它们可以清除 ROS，并在分化过程中发挥重要的信号作用。
