Pan Hong-Hui, Yu Xiong-Tao, Li Ting, Wu Hong-Ling, Jiao Chun-Wei, Cai Mian-Hua, Li Xiang-Min, Xie Yi-Zhen, Wang Yi, Peng Tao
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology, South China, Guangzhou, China.
Int J Med Mushrooms. 2013;15(1):29-38. doi: 10.1615/intjmedmushr.v15.i1.40.
Chaga medicinal mushroom, Inonotus obliquus, a popular prescription in traditional medicine in Europe and Asia, was used to reduce inflammation in the nasopharynx and to facilitate breathing. The aqueous extract from I. obliquus (AEIO) exhibited marked decrease in herpes simplex virus (HSV) infection (the 50% inhibitory concentration was 3.82 μg/mL in the plaque reduction assay and 12.29 μg/mL in the HSV-1/blue assay) as well as safety in Vero cells (the 50% cellular cytotoxicity was > 1 mg/mL, and selection index was > 80). Using a time course assay, effective stage analysis, and fusion inhibition assay, the mechanism of anti-HSV activity was found against the early stage of viral infection through inhibition of viral-induced membrane fusion. Therefore, AEIO could effectively prevent HSV-1 entry by acting on viral glycoproteins, leading to the prevention of membrane fusion, which is different from nucleoside analog antiherpetics.
桦褐孔菌，又称斜顶孔菌，是一种在欧洲和亚洲传统医学中常用的药材，用于减轻鼻咽部炎症并促进呼吸。桦褐孔菌水提取物（AEIO）对单纯疱疹病毒（HSV）感染有显著抑制作用（在蚀斑减少试验中50%抑制浓度为3.82μg/mL，在HSV-1/蓝色试验中为12.29μg/mL），且对Vero细胞具有安全性（50%细胞毒性>1mg/mL，选择指数>80）。通过时间进程试验、有效阶段分析和融合抑制试验，发现AEIO抗HSV活性的机制是通过抑制病毒诱导的膜融合作用于病毒感染的早期阶段。因此，AEIO可通过作用于病毒糖蛋白有效阻止HSV-1进入，从而防止膜融合，这与核苷类似物抗疱疹药不同。
