Yisraeli J K, Sokol S, Melton D A
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Development. 1990 Feb;108(2):289-98. doi: 10.1242/dev.108.2.289.
In an effort to understand how polarity is established in Xenopus oocytes, we have analyzed the process of localization of the maternal mRNA, Vg1. In fully grown oocytes, Vg1 mRNA is tightly localized at the vegetal cortex. Biochemical fractionation shows that the mRNA is preferentially associated with a detergent-insoluble subcellular fraction. The use of cytoskeletal inhibitors suggests that (1) microtubules are involved in the translocation of the message to the vegetal hemisphere and (2) microfilaments are important for the anchoring of the message at the cortex. Furthermore, immunohistochemistry reveals that a cytoplasmic microtubule array exists during translocation. These results suggest a role for the cytoskeleton in localizing information in the oocyte.
为了了解非洲爪蟾卵母细胞中极性是如何建立的，我们分析了母源mRNA Vg1的定位过程。在完全成熟的卵母细胞中，Vg1 mRNA紧密定位于植物性皮质。生化分级分离表明，该mRNA优先与去污剂不溶性亚细胞组分相关。细胞骨架抑制剂的使用表明：（1）微管参与了信息向植物性半球的转运；（2）微丝对于信息在皮质的锚定很重要。此外，免疫组织化学显示在转运过程中存在细胞质微管阵列。这些结果表明细胞骨架在卵母细胞中定位信息方面发挥了作用。
