Malatya EGM Hayat Hospital, Ophthalmology Clinic, Malatya, Turkiye.
Ocul Immunol Inflamm. 2013 Jun;21(3):197-200. doi: 10.3109/09273948.2012.758747. Epub 2013 Mar 20.
To determine the differences between conjunctival bacterial flora of hemodialysis patients and healthy subjects, the effect of duration of hemodialysis on conjunctival bacterial flora, and in vitro antibiotic susceptibility patterns of conjunctival bacterial flora to fluoroquinolones.
Sixty healthy subjects (32 male, 28 female) and 60 hemodialysis patients (33 male, 27 female) who had renal failure due to reasons other than diabetic nephropathy and with no previous history of intraocular surgery participated in this study. Statistical analysis of the difference between the culture positivity of hemodialysis patients and a control group was determined using the chi-square test. Pearson's correlation test was used to quantify the correlations among culture positivity and duration of hemodialysis.
Conjunctival culture positivity of the hemodialysis patients and the control group was 58.3 and 45.0%, respectively, and there was no statistically significant difference between these groups (p = 0.144). Pearson's correlation test revealed that there was a weak but statistically significant positive correlation between culture positivity and duration of hemodialysis (r value was 0.378 [p = 0.003]). Staphylococcus aureus (28.3%) and Staphylococcus epidermidis (20.0%) were the most frequently isolated organisms in the hemodialysis patients, Staphylococcus epidermidis (26.7%) and Staphylococcus aureus (6.7%) were the most frequently isolated organisms in the control group. Resistance of most frequently isolated organisms (Staphylococcus aureus and Staphylococcus epidermidis) from control group and hemodialysis patients to ofloxacin, levofloxacin, moxifloxacin, and gatifloxacin were 20, 15, 5, and 5%, and 34.5, 20.7, 6.9, and 6.9%, respectively.
As distinct from the control group, S. aureus colonization was isolated more from the hemodialysis patients. Culture positivity of the hemodialysis patients was positively correlated with the duration of hemodialysis. Antibiotic susceptibility of isolates from conjunctival bacterial flora of hemodialysis patients were less than healthy control group.
确定血液透析患者与健康受试者之间结膜细菌菌群的差异、血液透析持续时间对结膜细菌菌群的影响，以及结膜细菌菌群对氟喹诺酮类药物的体外抗生素敏感性模式。
本研究纳入了 60 名健康受试者（男 32 名，女 28 名）和 60 名因非糖尿病肾病导致肾衰竭且无眼部手术史的血液透析患者（男 33 名，女 27 名）。使用卡方检验对血液透析患者和对照组的培养阳性率差异进行统计学分析。采用皮尔逊相关检验量化培养阳性率与血液透析持续时间之间的相关性。
血液透析患者和对照组的结膜培养阳性率分别为 58.3%和 45.0%，两组之间无统计学差异（p=0.144）。皮尔逊相关检验显示，培养阳性率与血液透析持续时间之间存在弱但具有统计学意义的正相关（r 值为 0.378 [p=0.003]）。血液透析患者中最常分离的病原体是金黄色葡萄球菌（28.3%）和表皮葡萄球菌（20.0%），对照组中最常分离的病原体是表皮葡萄球菌（26.7%）和金黄色葡萄球菌（6.7%）。对照组和血液透析患者中最常分离的病原体（金黄色葡萄球菌和表皮葡萄球菌）对氧氟沙星、左氧氟沙星、莫西沙星和加替沙星的耐药率分别为 20%、15%、5%和 5%，34.5%、20.7%、6.9%和 6.9%。
与对照组不同，血液透析患者中分离出更多的金黄色葡萄球菌定植。血液透析患者的培养阳性率与血液透析持续时间呈正相关。血液透析患者结膜细菌菌群分离株的抗生素敏感性低于健康对照组。
