Kusbeci Tuncay, Kusbeci Ozge Yilmaz, Aktepe Orhan Cem, Yavas Güliz, Ermis S Samet
Department of Ophthalmology, Kocatepe University School of Medicine, Afyonkarahisar, Turkey.
Curr Eye Res. 2009 Apr;34(4):251-6. doi: 10.1080/02713680902725970.
To identify the conjunctival flora in patients with Parkinson's disease (PD) and compare it with the conjunctival flora of healthy subjects.
One hundred six eyes of 106 patients with PD and 102 eyes of 102 age-matched healthy subjects were included in the study. All subjects had a detailed anterior segment examination, including eye blink rate and conjunctival cultures. Conjunctival cultures were taken with a sterile cotton-tipped dry swab without topical anesthesia. The culture samples were inoculated onto 5% sheep blood agar and chocolate agar, as well as brain heart infusion broth. The bacterial growth was evaluated in a quantitative manner as colony-forming units (CFU). All bacterial isolates were identified, and statistical analyses were performed by chi-square test to determine if there were differences in flora between PD and control groups.
Eighty-six (81.1%) of the 106 eyes in patients with PD and 73 (71.5%) of the 102 eyes in control group were found to have positive conjunctival cultures (p = 0.144). Coagulase-negative Staphylococcus was the most commonly isolated bacterial species in both groups, found in 45 (42.4%) and 49 (48%) of eyes in patients with PD and control group, respectively (p = 0.503). Among all bacterial isolates, only Staphylococcus aureus was found to be statistically different between the two groups, noted in 32 (30.1%) and 10 (9.8%) of eyes in patients with PD and control group, respectively (p = 0.001).
Coagulase-negative Staphylococcus, Staphylococcus aureus, and Corynebacterium species were the most commonly isolated bacterial species in the PD group. Among all bacterial isolates, only Staphylococcus aureus was significantly higher in the conjunctival flora of patients with PD than in those in the control group.
识别帕金森病（PD）患者的结膜菌群，并将其与健康受试者的结膜菌群进行比较。
本研究纳入了106例PD患者的106只眼和102例年龄匹配的健康受试者的102只眼。所有受试者均进行了详细的眼前节检查，包括眨眼率和结膜培养。结膜培养采用无菌棉拭子，不进行表面麻醉。将培养样本接种到5%羊血琼脂、巧克力琼脂以及脑心浸液肉汤中。以菌落形成单位（CFU）对细菌生长进行定量评估。鉴定所有细菌分离株，并通过卡方检验进行统计分析，以确定PD组和对照组之间的菌群是否存在差异。
PD患者的106只眼中有86只（81.1%）结膜培养呈阳性，对照组的102只眼中有73只（71.5%）结膜培养呈阳性（p = 0.144）。凝固酶阴性葡萄球菌是两组中最常分离出的细菌种类，分别在PD患者组和对照组的45只眼（42.4%）和49只眼（48%）中发现（p = 0.503）。在所有细菌分离株中，仅金黄色葡萄球菌在两组之间存在统计学差异，分别在PD患者组和对照组的32只眼（30.1%）和10只眼（9.8%）中发现（p = 0.001）。
凝固酶阴性葡萄球菌、金黄色葡萄球菌和棒状杆菌属是PD组中最常分离出的细菌种类。在所有细菌分离株中，仅PD患者结膜菌群中的金黄色葡萄球菌显著高于对照组。
