Hosokawa N, Hosokawa Y, Sakai T, Yoshida M, Marui N, Nishino H, Kawai K, Aoike A
Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Japan.
Int J Cancer. 1990 Jun 15;45(6):1119-24. doi: 10.1002/ijc.2910450622.
Quercetin inhibits growth of COLO320 DM cells, derived from a human colon cancer. The inhibitory effect is partially reversible when quercetin is removed from the culture medium. Flow cytometric analysis has revealed that quercetin causes perturbation of the cell cycle, inducing a frozen cell-cycle pattern and a block at the G1/S boundary. The synthesis of a 17-kDa protein was specifically inhibited by the addition of quercetin, and recovered when the cells at the G1/S boundary progressed into S-phase after the removal of quercetin from the culture medium. Furthermore, using synchronized cells obtained by centrifugal elutriation, we have shown that the rate of synthesis of a 17-kDa protein was low in G1, and high in S-phase of the cell cycle. Thus, this protein appears to be cell-cycle-related.
槲皮素可抑制源自人结肠癌的COLO320 DM细胞的生长。当从培养基中去除槲皮素时，这种抑制作用部分可逆。流式细胞术分析显示，槲皮素会扰乱细胞周期，诱导细胞周期模式冻结并在G1/S边界处发生阻滞。添加槲皮素会特异性抑制17 kDa蛋白的合成，当从培养基中去除槲皮素后，处于G1/S边界的细胞进入S期时，该蛋白的合成会恢复。此外，利用通过离心淘析获得的同步化细胞，我们发现17 kDa蛋白的合成速率在细胞周期的G1期较低，而在S期较高。因此，这种蛋白似乎与细胞周期相关。
