Robertson B
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Histochemistry. 1990;94(1):81-5. doi: 10.1007/BF00266793.
The binding of wheat germ agglutinin (WGA) to L5 dorsal root ganglion (DRG) cells in the rat was studied with the WGA-FITC conjugate. These cells were also examined with regard to overlap between WGA staining and choleragenoid-like immunoreactivity. The DRG cells showed varying intensity of the staining, which was confined to the cytoplasm. The majority of the small cells were heavily stained, whereas the large cells showed less or occasionally no staining. Lectin binding was observed along nerve fibres in the ganglion, and appeared to be localized to the Schwann cells and to the nodes of Ranvier. Strong staining was also observed in the area surrounding the ganglion cells and seemed to be confined to the satellite cells. A subpopulation of the ganglion cells showed both WGA-staining and choleragenoid-like immunoreactivity. These results indicate a nonuniform affinity of WGA for different subpopulations of DRG neurons.
用WGA-FITC偶联物研究了小麦胚芽凝集素（WGA）与大鼠L5背根神经节（DRG）细胞的结合。还对这些细胞进行了WGA染色与类霍乱毒素样免疫反应性重叠方面的检查。DRG细胞显示出不同强度的染色，染色局限于细胞质。大多数小细胞染色较深，而大细胞染色较少或偶尔无染色。在神经节内的神经纤维上观察到凝集素结合，似乎定位于施万细胞和郎飞结。在神经节细胞周围区域也观察到强染色，似乎局限于卫星细胞。神经节细胞的一个亚群同时显示WGA染色和类霍乱毒素样免疫反应性。这些结果表明WGA对DRG神经元不同亚群的亲和力不一致。
