Robertson B, Grant G
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
J Neurocytol. 1989 Feb;18(1):77-86. doi: 10.1007/BF01188426.
Localization of GM1 ganglioside, the receptor for cholera toxin, and choleragenoid, which is the binding subunit of cholera toxin, was studied in the rat L5 dorsal root ganglion. Sections were incubated with choleragenoid and treated immunocytochemically. Choleragenoid-like immunoreactive cells were then examined for possible co-localization with carbonic anhydrase-like, RT 97 (antibody to neurofilament proteins), substance P-like, somatostatin-like and calcitonin gene-related peptide-like immunoreactivity and fluoride-resistant acid phosphatase (FRAP) activity, using adjacent sections. A subpopulation of dorsal root ganglion neurons exhibited choleragenoid-like immunoreactivity. The majority of these were medium-sized and large neurons. The strongest immunoreactivity was found in the area of the plasma membrane, but strong reactivity was also seen in the cytoplasm. The majority of the choleragenoid-like immunoreactive cells showed carbonic anhydrase-like and RT 97 immunoreactivity. Cells showing co-localization of choleragenoid-like and neuropeptide-like immunoreactivity or activity for FRAP were rarely observed. Our results suggest that the GM1 receptor is localized primarily on carbonic anhydrase-containing and RT 97-immunoreactive primary sensory neurons.
在大鼠L5背根神经节中研究了霍乱毒素受体GM1神经节苷脂以及霍乱类毒素（霍乱毒素的结合亚基）的定位。将切片与霍乱类毒素一起孵育并进行免疫细胞化学处理。然后使用相邻切片，检查霍乱类毒素样免疫反应性细胞是否可能与碳酸酐酶样、RT 97（神经丝蛋白抗体）、P物质样、生长抑素样和降钙素基因相关肽样免疫反应性以及耐氟酸性磷酸酶（FRAP）活性共定位。背根神经节神经元的一个亚群表现出霍乱类毒素样免疫反应性。其中大多数是中等大小和大的神经元。在质膜区域发现最强的免疫反应性，但在细胞质中也可见强反应性。大多数霍乱类毒素样免疫反应性细胞显示出碳酸酐酶样和RT 97免疫反应性。很少观察到显示霍乱类毒素样和神经肽样免疫反应性或FRAP活性共定位的细胞。我们的结果表明，GM1受体主要定位于含碳酸酐酶和RT 97免疫反应性的初级感觉神经元上。
