State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China.
World J Microbiol Biotechnol. 2013 Sep;29(9):1585-95. doi: 10.1007/s11274-013-1322-4. Epub 2013 Mar 21.
The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.
肠道微生物群受到越来越多的关注，因为它影响生长、饲料转化率、上皮发育、免疫以及肠道中病原微生物的入侵。在这项研究中，我们使用焦磷酸测序技术来探索吉富罗非鱼（Carassius auratus gibelio）肠道的细菌群落及其微生物的起源。结果表明，罗非鱼肠道和培养环境中存在着巨大的细菌多样性。吉富罗非鱼具有特征性的肠道微生物群，其中变形菌门（Proteobacteria）占优势，其次是厚壁菌门（Firmicutes）。对 10 个最丰富的细菌操作分类单元（OTUs）的分析表明，肠道内容物中以厚壁菌门为主（递减顺序为：韦荣球菌属（Veilonella sp.）、毛螺菌科（Lachnospiraceae）、乳杆菌目（Lactobacillales）、链球菌属（Streptococcus sp.）和乳杆菌属（Lactobacillus sp.））。第二丰富的 OTU 是罗特氏菌属（Actinobacteria）。最有可能的益生菌（乳杆菌属（Lactobacillus sp.）和芽孢杆菌属（Bacillus sp.））和机会主义者（气单胞菌属（Aeromonas sp.）和不动杆菌属（Acinetobacter sp.））并不丰富。细菌群落比较表明，肠道群落与沉积物密切相关，这表明沉积物作为吉富罗非鱼肠道细菌的来源非常重要。然而，在肠道中检测到的 37.95%的 OTUs 存在于饲料中，这表明食物可能会显著影响吉富罗非鱼的微生物群，因此可以利用食物来进行益生菌的口服给药。
