College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China.
The Station of Fishery Management, Yiwu City Water Conservancy Bureau of Zhejiang Province, Yiwu, 322000, China.
Probiotics Antimicrob Proteins. 2017 Dec;9(4):386-396. doi: 10.1007/s12602-017-9269-7.
Gut microbiota of grass carp plays an important role in host. However, detailed information regarding the changes of microbiota after probiotics administration in relation to the gastrointestinal microbiota is absent. In the present study, dietary administration of putative probiotics Shewanella xiamenensis A-1, Aeromonas veronii A-7, and Bacillus subtilisstrains was conducted in grass carp to investigate if there is a discernible alteration in intestinal microbiota and whether the alteration is associated with previous study about the immunity regulation in grass carp. Bacterial 16S rRNA gene sequence-based comparisons of the bacterial communities in the grass carp intestine were detected after 28 days feeding by five diets, and results demonstrated the changes of microbial community composition at genus level. The abundance of Cetobacterium genus with potential immunity function increased. Potential pathogens and probiotics are important constitutions of the intestinal microbiota. Orally taken probiotics considerably reduced the abundance of the potential pathogenic bacteria (e.g., Pseudomonas and Flavobacterium genus) in the intestine. Meanwhile, putative probiotics used in this study were favorable to the reproduction of potential probiotics in THE intestine of grass carp (e.g., Vibrio, Streptococcus, and Enterococcus genus). Moreover, modulation of intestinal environment by the probiotics could impact the abundance of cellulose-degrading bacteria (e.g., Citrobacter genus). Those results suggested that oral probiotics administration can positively improve the composition of intestinal microbial community in grass carp, and this was associated with regulation of immunity in grass carp. Probiotics-induced alteration of microbiota may potentially lower the risk of disease outbreaks during cultivation stage of grass carp.
草鱼肠道中的微生物群落在宿主中起着重要作用。然而，关于益生菌给药后与胃肠道微生物群相关的微生物群变化的详细信息尚不清楚。在本研究中，通过给草鱼投喂潜在益生菌厦门希瓦氏菌 A-1、维氏气单胞菌 A-7 和枯草芽孢杆菌，研究肠道微生物群是否发生了明显变化，以及这种变化是否与草鱼免疫调节的先前研究有关。在 28 天的 5 种不同饮食喂养后，通过细菌 16S rRNA 基因序列比较检测草鱼肠道中的细菌群落，结果表明在属水平上微生物群落组成发生了变化。具有潜在免疫功能的鲸杆菌属的丰度增加。潜在的病原体和益生菌是肠道微生物群的重要组成部分。口服益生菌可显著降低肠道中潜在致病菌（如假单胞菌属和黄杆菌属）的丰度。同时，本研究中使用的潜在益生菌有利于草鱼肠道中潜在益生菌（如弧菌属、链球菌属和肠球菌属）的繁殖。此外，益生菌对肠道环境的调节可能会影响纤维素降解菌（如柠檬酸杆菌属）的丰度。这些结果表明，口服益生菌给药可以积极改善草鱼肠道微生物群落的组成，这与草鱼的免疫调节有关。益生菌诱导的微生物群变化可能会降低草鱼养殖阶段疾病爆发的风险。
