Brooks J B, Daneshvar M I, Haberberger R L, Mikhail I A
Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1990 May;28(5):989-97. doi: 10.1128/jcm.28.5.989-997.1990.
The frequency-pulsed electron-capture gas-liquid chromatography technique described previously by Brooks et al. was modified and applied to the studies of coded and routine clinical specimens. Uncentrifuged cerebrospinal fluid (2 ml) was extracted under acidic conditions, derivatized, and analyzed by frequency-pulsed electron-capture gas-liquid chromatography on large-bore fused silica polar and nonpolar capillary columns. The frequency-pulsed electron-capture gas-liquid chromatography profile of carboxylic acids (C2 through C22) along with identification of tuberculostearic acid, established by retention time comparison of derivatized tuberculostearic acid and derivatized sample extract, strongly suggests the presence of Mycobacterium tuberculosis in patients with lymphocytic meningitis. Results from 41 coded cases and 75 clinical cases showed that the frequency-pulsed electron-capture gas-liquid chromatography test had a specificity of 91% and a sensitivity of 95%.
布鲁克斯等人之前描述的频率脉冲电子捕获气液色谱技术经过改进后应用于编码临床标本和常规临床标本的研究。未离心的脑脊液(2毫升)在酸性条件下进行提取、衍生化处理,然后在大口径熔融石英极性和非极性毛细管柱上通过频率脉冲电子捕获气液色谱进行分析。通过衍生化结核硬脂酸与衍生化样品提取物的保留时间比较确定的羧酸(C2至C22)的频率脉冲电子捕获气液色谱图谱,有力地表明淋巴细胞性脑膜炎患者中存在结核分枝杆菌。41例编码病例和75例临床病例的结果显示,频率脉冲电子捕获气液色谱检测的特异性为91%,灵敏度为95%。