Caimi Gregorio, Hopps Eugenia, Noto Davide, Canino Baldassare, Montana Maria, Lucido Daniela, Lo Presti Rosalia, Averna Maurizio R
Dipartimento di Medicina Interna e Specialistica, Università di Palermo, Italy.
Diabetes Metab Syndr. 2013 Jan-Mar;7(1):38-41. doi: 10.1016/j.dsx.2013.02.013. Epub 2013 Mar 13.
To examine the protein oxidation, marker of the oxidative stress, in metabolic syndrome (MS).
We enrolled 106 subjects (45 women and 61 men) with MS of which 43 (14 women and 27 men) were with diabetes mellitus and 63 (31 women and 32 men) were without diabetes mellitus, and 54 subjects (19 women and 35 men) as control group. The protein oxidation, expressed as carbonyl groups, was measured by an enzyme-like immunosorbent assay (ELISA) kit (BioCell PC test kit, Enzo Life Sciences AG, Switzerland).
In the whole group of MS subjects, in comparison with control group, a significant increase in carbonyl groups was present. The same datum was also evident between control group and diabetic subjects with MS and between control group and nondiabetic subjects with MS. No difference was observed between the two subgroups (diabetic and nondiabetic subjects with MS) about NOx. Few information were obtained examining the linear regression among carbonyl groups, age, BMI, waist circumference, blood pressure values and metabolic pattern of MS subjects.
In MS subject we observed an increase of protein oxidation not influenced by diabetes mellitus. Several strategies may be employed to reduce this parameter.
研究代谢综合征（MS）中氧化应激标志物——蛋白质氧化情况。
我们招募了106名患有MS的受试者（45名女性和61名男性），其中43名（14名女性和27名男性）患有糖尿病，63名（31名女性和32名男性）未患糖尿病，以及54名受试者（19名女性和35名男性）作为对照组。通过酶联免疫吸附测定（ELISA）试剂盒（BioCell PC检测试剂盒，瑞士恩佐生命科学股份公司）测量以羰基表示的蛋白质氧化。
在整个MS受试者组中，与对照组相比，羰基显著增加。对照组与患有MS的糖尿病受试者之间以及对照组与患有MS的非糖尿病受试者之间也有相同的数据。在两个亚组（患有MS的糖尿病和非糖尿病受试者）之间未观察到关于NOx的差异。在研究MS受试者的羰基、年龄、体重指数、腰围、血压值和代谢模式之间的线性回归时，获得的信息很少。
在MS受试者中，我们观察到蛋白质氧化增加，且不受糖尿病影响。可以采用多种策略来降低这一参数。
