Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Zagazig, Zagazig, Egypt.
East Mediterr Health J. 2013 Jan;19(1):71-80.
This study aimed to characterize Pseudomonas aeruginosa isolates in 2 intensive care units in Egypt and Saudi Arabia. P. aeruginosa isolates from patients' and staff hands and environmental samples were typed using antibiotyping and ERIC-PCR. In Egypt, isolates from suction apparatus tubing and drainage containers (A7) and AV tubing (A8) were linked to those from patients who had these antibiotypes. In Saudi Arabia, isolates from suction apparatus tubing (A6) and AV tubing (A7) were linked to patients with the same antibiotypes. In Egypt, patients' isolates had ERIC VII, VIll and IX patterns linked to suction apparatus tubing, AV machine tubes and drainage containers. In Saudi Arabia, patients' isolates had ERIC VIII and XI patterns linked to suction apparatus tubing and AV machines. In Egypt and Saudi Arabia, ERIC typing gave higher discriminatory indices (0.801 and 0.785 respectively) than the antibiotyping (0.7123 and 0.728 respectively). ERIC was superior to antibiotyping and should be used in tracing sources of infection.
本研究旨在描述埃及和沙特阿拉伯 2 家重症监护病房（ICU）中的铜绿假单胞菌分离株。采用抗生素分型和 ERIC-PCR 对来自患者和医护人员手部及环境样本的铜绿假单胞菌分离株进行分型。在埃及，来自吸引装置管和引流容器（A7）和 AV 管（A8）的分离株与具有这些抗生素型的患者分离株相关联。在沙特阿拉伯，来自吸引装置管（A6）和 AV 管（A7）的分离株与具有相同抗生素型的患者相关联。在埃及，患者分离株具有与吸引装置管、AV 机器管和引流容器相关联的 ERIC VII、VIII 和 IX 型。在沙特阿拉伯，患者分离株具有与吸引装置管和 AV 机器相关联的 ERIC VIII 和 XI 型。在埃及和沙特阿拉伯，ERIC 分型的区分指数（分别为 0.801 和 0.785）高于抗生素分型（分别为 0.7123 和 0.728）。ERIC 优于抗生素分型，应用于追踪感染源。
