Al Dawodeyah Heba Y, Obeidat Nathir, Abu-Qatouseh Luay F, Shehabi Asem A
MSc, Department of Pathology-Microbiology, School of Medicine, The Jordan University, Queen Rania str. 100, Amman, Jordan.
MD, Department of Internal Medicine/Division of Pulmonary Diseases, The Jordan University Hospital, Queen Rania str. 100, Amman, Jordan.
Germs. 2018 Mar 3;8(1):31-40. doi: 10.18683/germs.2018.1130. eCollection 2018 Mar.
is a common agent causing community acquired and nosocomial respiratory tract infections, with particularly life-threatening manifestations in patients who are immunocompromised of who have cystic fibrosis. This study investigated the occurrence of extended-spectrum β-lactamases (ESBLs) and metallo β-lactamase (MBL) in association with important putative virulence genes and genotypes variation among isolates from respiratory tract infection of Jordanian patients.
Over a period of 8-month, a total of 284 respiratory tract samples were obtained from patients diagnosed with respiratory tract infection while attending the Pulmonary Clinic/Intensive Care Unit, Jordan University Hospital (JUH). At the time of sampling most were inpatients (86.9%). Samples were cultured specifically for .
A total of 61/284 (21.5%) isolates were recovered from respiratory samples of patients. The percentage of MDR isolates was 52.5%, and all isolates were susceptible to colistin with lower rates of susceptibility to other tested antibiotics. Positive genes of , , , and were detected in 68.9%, 18.9%, 18.9%, 15.6% and 12.5% of isolates, respectively. Genotyping revealed no significant genetic relationship among MDR isolates from hospitalized patients as judged by the constructed dendrogram and the presence of 14 genotypic groups. The percentages of the virulence genes , , , , and among isolates were 98%, 98%, 80%, 33% and 33%, respectively, and 87% of isolates produced pyocyanin.
The present study demonstrates high occurrence of MDR isolates carrying genes. No specific associations were found between antibiotic resistance, virulence genes and genotypes among MDR isolates.
是引起社区获得性和医院获得性呼吸道感染的常见病原体，在免疫功能低下或患有囊性纤维化的患者中可导致特别危及生命的表现。本研究调查了约旦患者呼吸道感染分离株中与重要假定毒力基因相关的超广谱β-内酰胺酶（ESBLs）和金属β-内酰胺酶（MBL）的发生情况以及基因型变异。
在8个月的时间里，从约旦大学医院（JUH）肺科诊所/重症监护病房诊断为呼吸道感染的患者中总共采集了284份呼吸道样本。采样时大多数患者为住院患者（86.9%）。样本专门培养用于。
从患者的呼吸道样本中总共分离出61/284（21.5%）株。多重耐药株的比例为52.5%，所有分离株对黏菌素敏感，对其他测试抗生素的敏感率较低。分别在68.9%、18.9%、18.9%、15.6%和12.5%的分离株中检测到、、、和的阳性基因。基因分型显示，根据构建的树状图和14个基因型组的存在情况判断，住院患者的多重耐药株之间没有显著的遗传关系。分离株中毒力基因、、、、和的比例分别为98%、98%、80%、33%和33%，87%的分离株产生绿脓菌素。
本研究表明携带基因的多重耐药株发生率很高。在多重耐药株中未发现抗生素耐药性、毒力基因和基因型之间的特定关联。
