Department of Neurology, University of Utah, Salt Lake City, UT 84132, USA.
J Peripher Nerv Syst. 2013 Mar;18(1):54-8. doi: 10.1111/jns5.12008.
In order to develop an efficient, reproducible, and well-tolerated protocol for assessing corneal innervation, 11 normal subjects underwent corneal confocal microscopy (CCM) using a Heidelberg Retinal Tomography III microscope. Five standardized locations were sampled in the left eye and one centrally in the right. The protocol was repeated 1-4 weeks later. A blinded technician measured nerve fiber length (NFL) and tortuosity coefficient (TC). The relationship between image location and NFL and TC was assessed using one-way analysis of variance, and reproducibility determined using relative intertrial variability and intraclass correlation coefficients. NFL reproducibility was maximized by averaging four or more images from the left eye, or one central image from both eyes. TC was less reproducible. CCM is a rapid, well-tolerated, and reproducible method for assessing corneal innervation.
为了开发一种高效、可重现且耐受良好的评估角膜神经支配的方案，11 名正常受试者接受了海德堡视网膜断层扫描仪 III 显微镜的角膜共焦显微镜（CCM）检查。左眼采集了 5 个标准位置，右眼采集了 1 个中央位置。1-4 周后重复该方案。一位盲法技术员测量了神经纤维长度（NFL）和扭曲系数（TC）。使用单因素方差分析评估图像位置与 NFL 和 TC 的关系，并使用相对试验间变异性和组内相关系数确定可重复性。通过平均左眼 4 个或更多图像，或双眼 1 个中央图像，可最大限度地提高 NFL 的可重复性。TC 的可重复性较差。CCM 是一种快速、耐受良好且可重现的评估角膜神经支配的方法。
