Dzegilenko N B, Korchagin V B, Zhdanovich I V, Petrakushina Z V
Antibiotiki. 1975 Feb;20(2):154-8.
A quantitative spectrophotmetric method for the assay of variamycin in commercial samples was developed. It was based on the measurement of the optical density of variamycin solutions in 0.01 N hydrochloric acid at a UV spectrum wave length of 412 nm. A method of thin-layer chromatography for a semi-quantitative estimation of tetraside, the main admixture of variamycin was devised. On storage for 18 months the content of variamycin in the preparations did not change as compared to that at the moment of the drug manufacturing and the amount of tetraside in 18 months did not exceed 2 per cent.
建立了一种用于测定市售样品中变霉素的定量分光光度法。该方法基于在0.01N盐酸中，于紫外光谱波长412nm处测量变霉素溶液的光密度。设计了一种薄层色谱法用于半定量估计变霉素的主要杂质四糖苷。制剂储存18个月后，变霉素的含量与药物生产时相比没有变化，且18个月后四糖苷的量不超过2%。
