Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. De Carpio y Plan de Ayala, Col. Sto. Tomas, Mexico, Distrito Federal, 11340, Mexico.
Microb Ecol. 2013 Jul;66(1):200-10. doi: 10.1007/s00248-013-0206-3. Epub 2013 Mar 24.
The bark beetles of the genus Dendroctonus feed on phloem that is a nitrogen-limited source. Nitrogen fixation and nitrogen recycling may compensate or alleviate such a limitation, and beetle-associated bacteria capable of such processes were identified. Raoultella terrigena, a diazotrophic bacteria present in the gut of Dendroctonus rhizophagus and D. valens, exhibited high acetylene reduction activity in vitro with different carbon sources, and its nifH and nifD genes were sequenced. Bacteria able to recycle uric acid were Pseudomonas fluorescens DVL3A that used it as carbon and nitrogen source, Serratia proteomaculans 2A CDF and Rahnella aquatilis 6-DR that used uric acid as sole nitrogen source. Also, this is the first report about the uric acid content in whole eggs, larvae, and adults (male and female) samples of the red turpentine beetle (Dendroctonus valens). Our results suggest that the gut bacteria of these bark beetles could contribute to insect N balance.
枝梢小蠹属的树皮甲虫以韧皮部为食，韧皮部是一种氮限制源。固氮和氮循环可能会补偿或缓解这种限制，并且已经鉴定出能够进行这些过程的与甲虫相关的细菌。在食菌沟胫天牛（Dendroctonus rhizophagus）和松墨天牛（D. valens）的肠道中存在好氧固氮菌拉乌尔菌（Raoultella terrigena），它在不同的碳源存在下具有很高的乙炔还原活性，并且其 nifH 和 nifD 基因被测序。能够回收尿酸的细菌有荧光假单胞菌 DVL3A，它将尿酸用作碳源和氮源，而鞘氨醇单胞菌 2A CDF 和海栖拉恩氏菌 6-DR 则将尿酸用作唯一的氮源。此外，这是首次报道红脂大小蠹（Dendroctonus valens）的全卵、幼虫和成虫（雄性和雌性）样本中的尿酸含量。我们的结果表明，这些树皮甲虫的肠道细菌可能有助于昆虫的氮平衡。
