Baños-Quintana Ana Patricia, Gershenzon Jonathan, Kaltenpoth Martin
Department of Insect Symbiosis, Max-Planck-Institute for Chemical Ecology, Jena, Germany.
Department of Biochemistry, Max-Planck-Institute for Chemical Ecology, Jena, Germany.
Front Microbiol. 2024 Feb 16;15:1367127. doi: 10.3389/fmicb.2024.1367127. eCollection 2024.
The Eurasian spruce bark beetle () is currently the most economically relevant pest of Norway spruce (). associates with filamentous fungi that may help it overcome the tree's chemical defenses. However, the involvement of other microbial partners in this pest's ecological success is unclear. To understand the dynamics of the bark beetle-associated microbiota, we characterized the bacterial and fungal communities of wild-collected and lab-reared beetles throughout their development by culture-dependent approaches, meta-barcoding, and quantitative PCR. Gammaproteobacteria dominated the bacterial communities, while the fungal communities were mainly composed of yeasts of the Saccharomycetales order. A stable core of microbes is shared by all life stages, and is distinct from those associated with the surrounding bark, indicating that influences the microbial communities of its environment and offspring. These findings coupled with our observations of maternal behavior, suggest that transfers part of its microbiota to eggs via deposition of an egg plug treated with maternal secretions, and by inducing an increase in abundance of a subset of taxa from the adjacent bark.
欧亚云杉树皮甲虫（）是目前对挪威云杉（）经济影响最为重大的害虫。它与丝状真菌共生，这些真菌可能有助于其克服树木的化学防御。然而，其他微生物伙伴在这种害虫生态成功中的作用尚不清楚。为了解与树皮甲虫相关的微生物群动态，我们通过依赖培养的方法、元条形码技术和定量PCR，对野生采集和实验室饲养的甲虫在其整个发育过程中的细菌和真菌群落进行了特征分析。γ-变形菌在细菌群落中占主导地位，而真菌群落主要由酵母目酵母菌组成。所有生命阶段都共享一个稳定的微生物核心，且该核心与周围树皮相关的微生物核心不同，这表明 会影响其环境和后代的微生物群落。这些发现以及我们对母性行为的观察表明， 通过用母体分泌物处理卵塞并诱导相邻树皮中一部分分类群的丰度增加，将其部分微生物群转移到卵中。
