用于化学生物技术的分子工具。
Molecular tools for chemical biotechnology.
机构信息
Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Department of Bioengineering, Stanford University, 473 Via Ortega, MC 4201, Stanford, CA 94305, United States.
出版信息
Curr Opin Biotechnol. 2013 Dec;24(6):1000-9. doi: 10.1016/j.copbio.2013.03.001. Epub 2013 Mar 23.
Biotechnological production of high value chemical products increasingly involves engineering in vivo multi-enzyme pathways and host metabolism. Recent approaches to these engineering objectives have made use of molecular tools to advance de novo pathway identification, tunable enzyme expression, and rapid pathway construction. Molecular tools also enable optimization of single enzymes and entire genomes through diversity generation and screening, whole cell analytics, and synthetic metabolic control networks. In this review, we focus on advanced molecular tools and their applications to engineered pathways in host organisms, highlighting the degree to which each tool is generalizable.
生物技术生产高价值化学产品越来越多地涉及工程体内多酶途径和宿主代谢。最近在这些工程目标上的方法利用分子工具来推进从头途径鉴定、可调酶表达和快速途径构建。分子工具还通过多样性产生和筛选、全细胞分析以及合成代谢控制网络来实现单个酶和整个基因组的优化。在这篇综述中，我们重点介绍了高级分子工具及其在宿主生物中的工程途径中的应用，突出了每种工具的通用性程度。
Zotero 插件