Orthopaedic Department, University of Duisburg-Essen, Germany; Orthopaedic Department, Heinrich-Heine-University Medical School, Düsseldorf, Germany.
J Biomed Mater Res A. 2013 Oct;101(10):2905-14. doi: 10.1002/jbm.a.34590. Epub 2013 Mar 25.
The fixation of cementless endoprostheses requires excellent fixation at the bone implant interface. Although the surface structures of these implants are designed to promote osteoblastic differentiation, poor bone quality may prevent or delay osseointegration. There is evidence that RGD peptides known as recognition motifs for various integrins, promote cellular adhesion, influence cellular proliferation, and differentiation of local cells. In this study, five different metal surfaces were analyzed: Sandblasted (TiSa) and polished (TiPol) Ti6Al4V, porocoated (CCPor) and polished (CCPol) cobalt chrome and polished stainless steel (SS) were coated by ethanol amine and poly(ethylene glycol) to attach covalently RGD peptides. Human mesenchymal stromal cells of healthy donors were cultivated onto prior functionalized metal surfaces for 14 days without osteogenic stimulation. Cell proliferation and differentiation were quantitatively evaluated for native (I), NaOH pre-activated (II), NaOH pre-activated, and PEG-coated (III) as well as for RGD (IV) coated surfaces. The RGD immobilization efficiency was analyzed by epi-fluorescence spectroscopy, cell morphology was documented by light and scanning electron microscopy. The RGD-binding efficiency was TiSa > TiPol > SS > CCPor > CCPol. RGD coated surfaces showed the highest average cell proliferation on CCPol > SS > CCPor > TiSa ≥ TiPol, whereas cellular differentiation mostly correlated with the observed proliferation results, such as CCPol > TiSa > SS > CCPor > TiPol. Considering statistical analyses (significance level of α = 0.05), the RGD-coating of all biometals in comparison and in respect of their particular controls showed no significant improvement in cellular proliferation and osteoblastic differentiation.
骨水泥型假体的固定需要在骨-植入物界面有良好的固定。尽管这些植入物的表面结构旨在促进成骨细胞分化，但较差的骨质可能会妨碍或延迟骨整合。有证据表明，作为各种整合素识别基序的 RGD 肽可促进细胞黏附，影响细胞增殖和局部细胞分化。在这项研究中，分析了五种不同的金属表面：喷砂（TiSa）和抛光（TiPol）Ti6Al4V、多孔涂层（CCPor）和抛光（CCPol）钴铬以及抛光不锈钢（SS），通过乙醇胺和聚乙二醇共价附着 RGD 肽。健康供体的人骨髓基质细胞在未进行成骨刺激的情况下培养于之前功能化的金属表面 14 天。定量评估了天然（I）、经 NaOH 预处理（II）、经 NaOH 预处理和 PEG 涂层（III）以及 RGD（IV）涂层表面的细胞增殖和分化。通过荧光显微镜分析 RGD 固定效率，用光镜和扫描电子显微镜记录细胞形态。RGD 结合效率为 TiSa > TiPol > SS > CCPor > CCPol。RGD 涂层表面在 CCPol > SS > CCPor > TiSa ≥ TiPol 上显示出最高的平均细胞增殖，而细胞分化主要与观察到的增殖结果相关，如 CCPol > TiSa > SS > CCPor > TiPol。考虑到统计分析（显著性水平α=0.05），与特定对照相比，所有生物金属的 RGD 涂层在细胞增殖和成骨分化方面没有显著改善。
