大鼠肝细胞组分中的碳酸氢根离子 - ATP酶

Bicarbonate ion-ATPase in rat liver cell fractions.

作者信息

Izutsu K T, Siegel I A

出版信息

Biochim Biophys Acta. 1975 Mar 13;382(2):193-203. doi: 10.1016/0005-2736(75)90177-7.

Abstract

The distribution of HCO3MINUS-ATPase activity was studied in cell fractions prepared from homogenates of rat liver. The level of mitochondrial contamination in the microsomal fraction depended on the fractionation procedure and on the method of homogenization. With proper care, microsomes with undetectable mitochondrial contamination could be prepared. These microsomes had no detectable HCO3MINUS-ATPase activity. Approximately 85% of the total HCO3minus-ATPase activity of the post 6000 times g-min supernatant was recovered in the mitochondrial fraction. The properties of this mitochondrial HCO3minus-ATPase were not distinguishable from those of the various microsomal HCO3minus-ATPases previously described by other investigators.

摘要

对从大鼠肝脏匀浆制备的细胞组分中的HCO₃⁻ -ATP酶活性分布进行了研究。微粒体组分中线粒体污染的程度取决于分级分离程序和匀浆方法。如果操作得当，可以制备出线粒体污染不可检测的微粒体。这些微粒体没有可检测到的HCO₃⁻ -ATP酶活性。6000倍重力离心后上清液中HCO₃⁻ -ATP酶总活性的约85%在线粒体组分中被回收。这种线粒体HCO₃⁻ -ATP酶的性质与其他研究者先前描述的各种微粒体HCO₃⁻ -ATP酶的性质没有区别。