Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Angew Chem Int Ed Engl. 2013 May 17;52(21):5571-4. doi: 10.1002/anie.201301362. Epub 2013 Mar 26.
A synthetic substrate enables a new colorimetric screen for terpene synthase cyclization activity, facilitating the engineering of these enzymes. Using directed evolution, the thermostability of the sesquiterpene synthase BcBOT2 was increased without the loss of other properties. The technique also enabled rapid optimization of conditions for expression and stabilization in lysate of another terpene synthase, SSCG_02150.
一种合成底物可实现新型显色法测定萜烯合酶环化活性，从而促进此类酶的工程化改造。通过定向进化，提高了倍半萜合酶 BcBOT2 的热稳定性，而没有损失其他特性。该技术还能够快速优化另一种萜烯合酶 SSCG_02150 在裂解物中表达和稳定的条件。
