模拟微重力对椎间盘退变的影响。
The effects of simulated microgravity on intervertebral disc degeneration.
机构信息
Orthopedic Research Laboratories, Department of Orthopedic Surgery, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
出版信息
Spine J. 2013 Mar;13(3):235-42. doi: 10.1016/j.spinee.2012.01.022.
BACKGROUND CONTEXT
Astronauts experience back pain, particularly low back pain, during and after spaceflight. Recent studies have described histologic and biochemical changes in rat intervertebral discs after space travel, but there is still no in vitro model to investigate the effects of microgravity on disc metabolism.
PURPOSE
To study the effects of microgravity on disc degeneration and establish an in vitro simulated microgravity study model.
STUDY DESIGN
Discs were cultured in static and rotating conditions in bioreactor, and the characteristics of disc degeneration were evaluated.
METHODS
The mice discs were cultured in a rotating wall vessel bioreactor where the microgravity condition was simulated. Intervertebral discs were cultured in static and microgravity condition. Histology, biochemistry, and immunohistochemical assays were performed to evaluate the characteristics of the discs in microgravity condition.
RESULTS
Intervertebral discs cultured in rotating bioreactors were found to develop changes of disc degeneration manifested by reduced red Safranin-O staining within the annulus fibrosus, downregulated glycosaminoglycan (GAG) content and GAG/hydroxyproline ratio, increased matrix metalloproteinase 3 expression, and upregulated apoptosis.
CONCLUSIONS
We conclude that simulated microgravity induces the molecular changes of disc degeneration. The rotating bioreactor model will provide a foundation to investigate the effects of microgravity on disc metabolism.
背景
宇航员在太空飞行期间和之后会经历背痛，特别是下背痛。最近的研究描述了太空旅行后大鼠椎间盘的组织学和生化变化，但仍没有体外模型来研究微重力对椎间盘代谢的影响。
目的
研究微重力对椎间盘退变的影响，并建立体外模拟微重力研究模型。
研究设计
椎间盘在生物反应器中进行静态和旋转培养，评估椎间盘退变的特征。
方法
在旋转壁容器生物反应器中模拟微重力条件，培养小鼠椎间盘。在静态和微重力条件下培养椎间盘。进行组织学、生物化学和免疫组织化学检测，评估微重力条件下椎间盘的特征。
结果
在旋转生物反应器中培养的椎间盘发生了退变变化，表现为纤维环内红色番红 O 染色减少，糖胺聚糖 (GAG) 含量和 GAG/羟脯氨酸比值降低，基质金属蛋白酶 3 表达增加，细胞凋亡增加。
结论
我们得出结论，模拟微重力诱导了椎间盘退变的分子变化。旋转生物反应器模型将为研究微重力对椎间盘代谢的影响提供基础。
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