School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
Sensors (Basel). 2013 Mar 28;13(4):4214-24. doi: 10.3390/s130404214.
In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.
在这项研究中，我们首次开发了一种基于两种异质大小金纳米粒子（Au NPs）探针的快速灵敏条带免疫传感器，用于检测饮用水中的痕量铅离子。在优化条件下，其灵敏度比传统的 LFA 高 4 倍。该放大方法用于定性检测铅离子的可见检测限（LOD）为 2ng/mL，使用扫描读数器进行半定量检测的 LOD 可降低至 0.19ng/mL。该方法不受其他金属离子的干扰，可用于检测饮用水中的痕量铅离子，无需样品富集。测试样品的回收率在 96%至 103%之间。由于检测方法可在 15 分钟内完成，因此该方法可用作初步监测饮用水中铅污染的潜在工具。
