固氮酶中铁钼辅因子的生物合成。
Biosynthesis of the iron-molybdenum cofactor of nitrogenase.
机构信息
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA.
出版信息
J Biol Chem. 2013 May 10;288(19):13173-7. doi: 10.1074/jbc.R113.454041. Epub 2013 Mar 28.
Abstract
The iron-molybdenum cofactor (the M-cluster) serves as the active site of molybdenum nitrogenase. Arguably one of the most complex metal cofactors in biological systems, the M-cluster is assembled through the formation of an 8Fe core prior to the insertion of molybdenum and homocitrate into this core. Here, we review the recent progress in the research area of M-cluster assembly, with an emphasis on our work that provides useful insights into the mechanistic details of this process.
摘要
铁-钼辅因子（M 簇）是钼氮酶的活性位点。可以说，M 簇是生物系统中最复杂的金属辅因子之一，它是通过在将钼和同型半胱氨酸插入该核心之前形成 8Fe 核心来组装的。在这里，我们回顾了 M 簇组装研究领域的最新进展，重点介绍了我们的工作，这些工作为深入了解该过程的机制细节提供了有用的见解。
相似文献
1
Biosynthesis of the iron-molybdenum cofactor of nitrogenase.固氮酶中铁钼辅因子的生物合成。
J Biol Chem. 2013 May 10;288(19):13173-7. doi: 10.1074/jbc.R113.454041. Epub 2013 Mar 28.
2
Biosynthesis of the iron-molybdenum cofactor of nitrogenase.固氮酶铁钼辅因子的生物合成
Annu Rev Microbiol. 2008;62:93-111. doi: 10.1146/annurev.micro.62.081307.162737.
3
Nitrogenase Cofactor Assembly: An Elemental Inventory.固氮酶辅因子组装：元素清单。
Acc Chem Res. 2017 Nov 21;50(11):2834-2841. doi: 10.1021/acs.accounts.7b00417. Epub 2017 Oct 24.
4
Structural Insights and Mechanistic Understanding of Iron-Molybdenum Cofactor Biosynthesis by NifB in Nitrogenase Assembly Process.结构洞察和氮酶组装过程中 NifB 对铁钼辅因子生物合成的机制理解。
Mol Cells. 2023 Dec 31;46(12):736-742. doi: 10.14348/molcells.2023.0140. Epub 2023 Nov 29.
5
Radical SAM-dependent formation of a nitrogenase cofactor core on NifB.NifB 上依赖于激进 SAM 的氮酶辅因子核心的形成。
J Inorg Biochem. 2022 Aug;233:111837. doi: 10.1016/j.jinorgbio.2022.111837. Epub 2022 Apr 20.
6
Assembly scaffold NifEN: A structural and functional homolog of the nitrogenase catalytic component.组装支架NifEN：固氮酶催化组分的结构和功能同源物。
Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9504-8. doi: 10.1073/pnas.1609574113. Epub 2016 Aug 9.
7
Formation of a homocitrate-free iron-molybdenum cluster on NifEN: implications for the role of homocitrate in nitrogenase assembly.在 NifEN 上形成无 homocitrate 的铁钼簇：对 homocitrate 在固氮酶组装中的作用的启示。
Dalton Trans. 2010 Mar 28;39(12):3124-30. doi: 10.1039/c000264j. Epub 2010 Feb 18.
8
Structure and Reactivity of an Asymmetric Synthetic Mimic of Nitrogenase Cofactor.氮酶辅因子的不对称合成模拟物的结构与反应性。
Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15633-15636. doi: 10.1002/anie.201608806. Epub 2016 Nov 10.
9
Biosynthesis of Nitrogenase Cofactors.固氮酶辅因子的生物合成。
Chem Rev. 2020 Jun 24;120(12):4921-4968. doi: 10.1021/acs.chemrev.9b00489. Epub 2020 Jan 24.
10
Cluster assembly in nitrogenase.固氮酶中的簇组装
Essays Biochem. 2017 May 9;61(2):271-279. doi: 10.1042/EBC20160071.
引用本文的文献
1
Ammonia synthesis via an engineered nitrogenase assembly pathway in .通过工程化固氮酶组装途径在……中合成氨
Nat Catal. 2024 Oct;7(10):1130-1141. doi: 10.1038/s41929-024-01229-x. Epub 2024 Sep 19.
2
NifEN: a versatile player in nitrogenase assembly, catalysis and evolution.NifEN：固氮酶组装、催化及进化过程中的多面手。
J Biol Inorg Chem. 2025 Mar;30(2):135-149. doi: 10.1007/s00775-024-02086-6. Epub 2024 Dec 12.
3
Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs.在两个 pH 值下对 apo 和钼酸盐结合的 ModA 的溶液 NMR 化学位移进行赋值。
Biomol NMR Assign. 2024 Jun;18(1):93-98. doi: 10.1007/s12104-024-10173-7. Epub 2024 Apr 20.
4
The Mechanisms of Molybdate Distribution and Homeostasis with Special Focus on the Model Plant .钼酸盐的分布和稳态机制，特别关注模式植物。
Molecules. 2023 Dec 20;29(1):40. doi: 10.3390/molecules29010040.
5
Heterologous synthesis of the complex homometallic cores of nitrogenase P- and M-clusters in .在.中异源合成氮酶 P 簇和 M 簇的复杂同金属核心
Proc Natl Acad Sci U S A. 2023 Oct 31;120(44):e2314788120. doi: 10.1073/pnas.2314788120. Epub 2023 Oct 23.
6
Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.).固氮酶蛋白在棉花（陆地棉）中的共表达。
PLoS One. 2023 Aug 24;18(8):e0290556. doi: 10.1371/journal.pone.0290556. eCollection 2023.
7
Rhamnolipid Enhances the Nitrogen Fixation Activity of by Influencing Lysine Succinylation.鼠李糖脂通过影响赖氨酸琥珀酰化增强[具体对象]的固氮活性。 （注：原文中“by Influencing Lysine Succinylation”前缺少具体对象，翻译时按字面意思补充了“[具体对象]”）
Front Microbiol. 2021 Jul 30;12:697963. doi: 10.3389/fmicb.2021.697963. eCollection 2021.
8
Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles.利用合成生物学克服真核细胞器中固氮酶稳定表达的障碍。
Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16537-16545. doi: 10.1073/pnas.2002307117. Epub 2020 Jun 29.
9
Reactivity, Mechanism, and Assembly of the Alternative Nitrogenases.交替固氮酶的反应性、机制和组装。
Chem Rev. 2020 Jun 24;120(12):5107-5157. doi: 10.1021/acs.chemrev.9b00704. Epub 2020 Mar 4.
10
The genome of Ensifer alkalisoli YIC4027 provides insights for host specificity and environmental adaptations.根瘤菌 YIC4027 的基因组为其宿主专一性和环境适应性提供了线索。
BMC Genomics. 2019 Aug 12;20(1):643. doi: 10.1186/s12864-019-6004-7.
本文引用的文献
1
Crystallographic structure and functional implications of the nitrogenase molybdenum-iron protein from azotobacter vinelandii.固氮酶钼铁蛋白的晶体结构及功能意义，来自维氏固氮菌。
Nature. 1992 Dec 10;360(6404):553-60. doi: 10.1038/360553a0.
2
X-ray spectroscopic observation of an interstitial carbide in NifEN-bound FeMoco precursor.X 射线能谱观察 NiFe 蛋白结合态 FeMoco 前体中的间隙碳化物。
J Am Chem Soc. 2013 Jan 16;135(2):610-2. doi: 10.1021/ja309254g. Epub 2013 Jan 8.
3
Radical SAM-dependent carbon insertion into the nitrogenase M-cluster.基于自由基S-腺苷甲硫氨酸的碳插入固氮酶M簇反应。
Science. 2012 Sep 28;337(6102):1672-5. doi: 10.1126/science.1224603.
4
X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor.X 射线发射光谱证据表明固氮酶铁钼辅因子中存在中心碳原子。
Science. 2011 Nov 18;334(6058):974-7. doi: 10.1126/science.1206445.
5
Evidence for interstitial carbon in nitrogenase FeMo cofactor.氮酶 FeMo 辅因子中存在间隙碳的证据。
Science. 2011 Nov 18;334(6058):940. doi: 10.1126/science.1214025.
6
Spectroscopic characterization of the isolated iron-molybdenum cofactor (FeMoco) precursor from the protein NifEN.从蛋白 NifEN 中分离的铁钼辅因子（FeMoco）前体的光谱特性。
Angew Chem Int Ed Engl. 2011 Aug 16;50(34):7787-90. doi: 10.1002/anie.201102724. Epub 2011 Jul 1.
7
NifEN-B complex of Azotobacter vinelandii is fully functional in nitrogenase FeMo cofactor assembly.固氮菌中非血红素铁蛋白 NifEN-B 复合物在固氮酶 FeMo 辅因子组装中完全功能化。
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8623-7. doi: 10.1073/pnas.1102773108. Epub 2011 May 5.
8
Structural basis for methyl transfer by a radical SAM enzyme.自由基 S-腺苷甲硫氨酸酶甲基转移的结构基础。
Science. 2011 May 27;332(6033):1089-92. doi: 10.1126/science.1205358. Epub 2011 Apr 28.
9
A radically different mechanism for S-adenosylmethionine-dependent methyltransferases.S-腺苷甲硫氨酸依赖型甲基转移酶的一种全新作用机制。
Science. 2011 Apr 29;332(6029):604-7. doi: 10.1126/science.1200877. Epub 2011 Mar 17.
10
Structure of precursor-bound NifEN: a nitrogenase FeMo cofactor maturase/insertase.前体结合态 NifEN 的结构：一种固氮酶 FeMo 辅因子成熟酶/插入酶。
Science. 2011 Jan 7;331(6013):91-4. doi: 10.1126/science.1196954.
Zotero 插件