Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
Bioprocess Biosyst Eng. 2013 Nov;36(11):1665-73. doi: 10.1007/s00449-013-0940-4. Epub 2013 Mar 31.
Different physiological and nutritional parameters affect the fermentative production of shikimic acid. In our study, Citrobacter freundii initially produced 0.62 g/L of shikimic acid in 72 h. However, when process optimization was employed, 5.11 g/L of shikimic acid was produced in the production medium consisting of glucose (5.0 %), asparagine (4.5 %), CaCO3 (2.0 %), at pH 6.0, when inoculated with 6 % inoculum and incubated at 30 ± 1 °C, 200 rpm for 60 h. Preliminary fed-batch studies have resulted in the production of 9.11 g/L of shikimic acid on feeding the production medium by 20 g/L of glucose at 24 h of the fermentation run. Production of similar amount of shikimic acid was observed when the optimized conditions were employed in a 10-L bioreactor as obtained in shake flask conditions. A total of 9.11 g/L of shikimic acid was produced in 60 h. This is approximately 14.69-fold increase in shikimic acid production when compared to the initial un-optimized production conditions. This has also resulted in the reduction of the production time. The present study provides useful information to the industrialists seeking environmentally benign technology for the production of bulk biomolecules through manipulation of various chemical parameters.
不同的生理和营养参数会影响莽草酸的发酵生产。在我们的研究中，最初在 72 小时内，弗氏柠檬酸杆菌生产了 0.62 g/L 的莽草酸。然而，当进行工艺优化时，在生产培养基中使用葡萄糖（5.0%）、天冬酰胺（4.5%）、CaCO3（2.0%），在 pH 值为 6.0、接种量为 6%、在 30±1°C、200 rpm 下培养 60 小时，可生产 5.11 g/L 的莽草酸。初步补料分批研究结果表明，在发酵运行 24 小时时，通过向生产培养基中添加 20 g/L 的葡萄糖，可以生产 9.11 g/L 的莽草酸。当在摇瓶条件下优化条件用于 10-L 生物反应器时，观察到类似量的莽草酸的生产。在 60 小时内共生产了 9.11 g/L 的莽草酸。与初始未优化的生产条件相比，莽草酸的产量增加了约 14.69 倍。这也缩短了生产时间。本研究为寻求通过操纵各种化学参数生产大量生物分子的环保技术的工业家提供了有用的信息。
