4-硫尿苷合成酶-RNA复合物的纯化、结晶及初步晶体学分析
Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex.
作者信息
Naumann Peter Thomas, Lauhon Charles T, Ficner Ralf
机构信息
Molecular Structural Biology, Georg-August-University Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):421-4. doi: 10.1107/S1744309113004077. Epub 2013 Mar 28.
Abstract
The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å.
摘要
硫转移酶4-硫尿苷合成酶（ThiI）参与了tRNA中U8的ATP依赖性修饰。克隆、过量表达并纯化了来自嗜热栖热菌的ThiI。制备并结晶了包含ThiI和截短tRNA的复合物，并收集了分辨率为3.5 Å的X射线衍射数据。晶体属于正交空间群P212121，晶胞参数为a = 102.9、b = 112.8、c = 132.8 Å。
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