Pizzoferrato L, Quattrucci E, Di Lullo G
Istituto Nazionale della Nutrizione, Rome, Italy.
Food Addit Contam. 1990 Mar-Apr;7(2):189-95. doi: 10.1080/02652039009373883.
A method based upon a combination of a modified Monier-Williams procedure and an HPLC separation and quantitation of sulphite, has been developed. Its efficiency has been tested, in comparison with the Monier-Williams method, for the SO2 recovery in various foods. Desorption of SO2 from different matrices is obtained by distillation in strongly acid solution. Gaseous SO2, collected and oxidized to sulphate with hydrogen peroxide, is neutralized with sodium hydroxide. The neutralized solutions are diluted and injected by an autosampling injector into an HPLC apparatus consisting of a strong anion exchange column, eluted with a potassium hydrogen phthalate solution (0.15 g/l, pH 5.7) at a 3 ml/min flow rate and detected at 280 nm by an UV spectrophotometer. The combined method shows a good detection limit as well as high chromatographic resolution, avoiding potential interference of other volatile compounds. It is also time-saving and utilized basic laboratory equipment.
已开发出一种基于改良的莫尼尔-威廉姆斯法与高效液相色谱法分离和定量亚硫酸盐相结合的方法。与莫尼尔-威廉姆斯法相比，已测试了该方法在各种食品中二氧化硫回收率方面的效率。通过在强酸溶液中蒸馏从不同基质中解吸出二氧化硫。收集气态二氧化硫并用过氧化氢将其氧化为硫酸盐，再用氢氧化钠中和。将中和后的溶液稀释，由自动进样器注入高效液相色谱仪，该色谱仪由强阴离子交换柱组成，用邻苯二甲酸氢钾溶液（0.15 g/l，pH 5.7）以3 ml/min的流速洗脱，并通过紫外分光光度计在280 nm处进行检测。该组合方法显示出良好的检测限以及高色谱分辨率，避免了其他挥发性化合物的潜在干扰。它还节省时间且使用的是基本的实验室设备。
