Dutia B M, Hopkins J, Allington M P, Bujdoso R, McConnell I
Department of Veterinary Pathology, University of Edinburgh, Summerhall.
Immunology. 1990 May;70(1):27-32.
In order to identify and characterize the sheep major histocompatibility complex (MHC) class II molecules, a panel of 19 monoclonal antibodies (mAb) has been raised following immunization of mice with a variety of class II antigen preparations. Antibodies were selected by ELISA using immunopurified sheep class II as antigen and further screened for the ability to react on immunoblots. Nine mAb reacted with the beta-chain and four reacted with the alpha-chain. The chain specificity of the remainder could not be determined as they did not blot. The anti-alpha and anti-beta mAb all reacted with deglycosylated class II. With the exception of one anti-alpha and one anti-beta antibody, the antibodies reacted with both efferent lymph cells and peripheral blood mononuclear cells (PBMC). Three of the anti-sheep class II mAb reacted with human PBMC.
为了鉴定和表征绵羊主要组织相容性复合体（MHC）II类分子，在用多种II类抗原制剂免疫小鼠后，制备了一组19种单克隆抗体（mAb）。通过酶联免疫吸附测定（ELISA），以免疫纯化的绵羊II类分子作为抗原筛选抗体，并进一步筛选其在免疫印迹上的反应能力。9种mAb与β链反应，4种与α链反应。其余抗体的链特异性无法确定，因为它们不出现印迹。抗α和抗β mAb均与去糖基化的II类分子反应。除一种抗α和一种抗β抗体外，这些抗体均与输出淋巴细胞和外周血单核细胞（PBMC）反应。三种抗绵羊II类mAb与人PBMC反应。
