Scott P C, Choi C L, Brandon M R
Immunogenetics. 1987;25(2):116-22. doi: 10.1007/BF00364277.
To study the class II genes of the major histocompatibility region of the sheep genome, human HLA class II genes corresponding to the known subregions in man (DR, DQ, DP, DO, and DZ) were used for Southern hybridization analysis of sheep DNA and to probe a sheep genomic library. Hybridizing bands were noted for all probes except DP alpha. DQ alpha and beta and DR beta appear to be present as multicopy genes, while DR alpha-, DZ alpha-, and DO beta- like genes appear to be single copy. All bands detected with the DP beta probe were also detectable with other beta chain probes. From eight lambda-bacteriophage clones of a sheep genomic library nine distinct class II genes were identified. These genes were characterized by differential hybridization analysis and restriction mapping. Two genes were DR beta-like, three DQ alpha-like and four DQ beta-like. The extensive cross-hybridization observed with beta chain probes was not seen with alpha chain probes. The results of this study suggest that the major histocompatibility complex class II region of the sheep has a similar genetic organization to that of man, with the provisional exception of the DP subregion.
为研究绵羊基因组主要组织相容性区域的Ⅱ类基因，将与人类已知亚区域（DR、DQ、DP、DO和DZ）相对应的人类HLAⅡ类基因用于绵羊DNA的Southern杂交分析，并用于筛选绵羊基因组文库。除DPα外，所有探针均观察到杂交条带。DQα和β以及DRβ似乎以多拷贝基因形式存在，而DRα、DZα和DOβ样基因似乎是单拷贝。用DPβ探针检测到的所有条带也能用其他β链探针检测到。从绵羊基因组文库的8个λ噬菌体克隆中鉴定出9个不同的Ⅱ类基因。这些基因通过差异杂交分析和限制性图谱进行表征。两个基因是DRβ样，三个是DQα样，四个是DQβ样。α链探针未观察到β链探针所观察到的广泛交叉杂交现象。本研究结果表明，绵羊的主要组织相容性复合体Ⅱ类区域具有与人类相似的遗传组织，临时排除DP亚区域。
