Goldberg I, Mateles R I
J Bacteriol. 1975 Apr;122(1):47-53. doi: 10.1128/jb.122.1.47-53.1975.
Pseudomonas C can grow on methanol, formaldehyde, or formate as sole carbon source. It is proposed that the assimilation of carbon by Pseudomonas C grown on different C1 growth substrates proceeds via one of two metabolic pathways, the serine pathway or the allulose pathway (the ribose phosphate cycle of formaldehyde fixation). This contention is based on the distribution of two key enzymes, each of which appears to be specifically involved in one of the assimilation pathways, glycerate dehydrogenase (serine pathway) and hexose phosphate synthetase (allulose pathway). The assimilation of methanol in Pseudomonas C cells appears to occur via the allulose pathway, whereas the utilization of formaldehyde or formate in cells grown on formaldehyde or formate as sole carbon sources appears by the serine pathway. When methanol is present together with formaldehyde or formate in the growth medium, the formaldehyde or formate is utilized by the allulose pathway.
假单胞菌C能够以甲醇、甲醛或甲酸盐作为唯一碳源生长。有人提出，在不同C1生长底物上生长的假单胞菌C对碳的同化作用是通过两种代谢途径之一进行的，即丝氨酸途径或阿洛酮糖途径（甲醛固定的磷酸核糖循环）。这一论点基于两种关键酶的分布情况，每种酶似乎都专门参与其中一种同化途径，即甘油酸脱氢酶（丝氨酸途径）和磷酸己糖合成酶（阿洛酮糖途径）。假单胞菌C细胞中甲醇的同化作用似乎是通过阿洛酮糖途径发生的，而在以甲醛或甲酸盐作为唯一碳源生长的细胞中，甲醛或甲酸盐的利用似乎是通过丝氨酸途径。当甲醇与甲醛或甲酸盐一起存在于生长培养基中时，甲醛或甲酸盐会通过阿洛酮糖途径被利用。
