Battat E, Goldberg I, Mateles R I
Appl Microbiol. 1974 Dec;28(6):906-11. doi: 10.1128/am.28.6.906-911.1974.
Pseudomonas C was grown in continuous culture on methanol, formaldehyde, or formate as sole carbon source. On methanol mu(max) = 0.49/h and yield constant (Y) = 0.54; on formaldehyde and on unsupplemented media, mu(max) was about 0.2/h and Y was 0.15, whereas addition of p-aminobenzoic acid, folic acid, serine, or glycine to the medium raised Y to about 0.26 to 0.29, and addition of p-aminobenzoic acid, folic acid, serine, nicotinamide adenine dinucleotide, and Tween 80 raised the yield to 0.35. On formate and on unsupplemented media, mu(max) = 0.2/h and Y = 0.02, whereas addition of 0.1 mM p-aminobenzoic acid increased mu(max) to about 0.47 and Y to about 0.23. At low cell concentrations or growth rates a beneficial effect of CO(2) was observed. Formaldehyde or formate, when added together with methanol, were utilized simultaneously with the methanol.
假单胞菌C在以甲醇、甲醛或甲酸盐作为唯一碳源的连续培养中生长。以甲醇为碳源时，最大比生长速率（μ(max)）= 0.49/h，产率常数（Y）= 0.54；以甲醛为碳源以及在未补充营养成分的培养基中，μ(max)约为0.2/h，Y为0.15，而向培养基中添加对氨基苯甲酸、叶酸、丝氨酸或甘氨酸可使Y提高至约0.26至0.29，同时添加对氨基苯甲酸、叶酸、丝氨酸、烟酰胺腺嘌呤二核苷酸和吐温80可使产率提高至0.35。以甲酸盐为碳源以及在未补充营养成分的培养基中，μ(max) = 0.2/h，Y = 0.02，而添加0.1 mM对氨基苯甲酸可使μ(max)提高至约0.47，Y提高至约0.23。在低细胞浓度或生长速率下，观察到二氧化碳具有有益作用。甲醛或甲酸盐与甲醇一起添加时，会与甲醇同时被利用。
