Khan Haroon, Saeed Muhammad, Muhammad Naveed, Gaffar Rukhsana, Gul Farah, Raziq Naila
Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
Toxicol Ind Health. 2015 Aug;31(8):758-63. doi: 10.1177/0748233713483197. Epub 2013 Apr 3.
Over expression of lipoxygenase (LOX) and urease has already contributed to the pathology of different human disease. Targeting the inhibition of these enzymes has proved great clinical utility. The aim of the present study was to scrutinised the inhibitory profile of the aerial parts of the Polygonatum verticillatum enzyme against LOX, urease, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) using standard experimental protocols. When checked against lipoxygenase, the extracts revealed significant attenuation. Of the tested extracts, the ethyl acetate fraction was the most potent (half-maximal inhibitory concentration (IC(50)): 97 µg/mL) followed by aqueous fraction IC(50): 109 µg/mL). Regarding urease inhibition, n-butanol was the most potent fraction (IC(50): 97 µg/mL). However, the extracts did not show significant inhibition on AChE and BChE. In the preliminary phytochemical tests, the aerial parts of the plant showed the presence of saponins, alkaloids, flavonoids, phenols, tannins and terpenoids. The current findings could be attributed to these groups of compounds.
脂氧合酶（LOX）和脲酶的过度表达已导致不同人类疾病的病理过程。针对这些酶的抑制作用已证明具有巨大的临床应用价值。本研究的目的是使用标准实验方案，仔细研究黄精地上部分对LOX、脲酶、乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制特性。当检测其对脂氧合酶的作用时，提取物显示出显著抑制作用。在测试的提取物中，乙酸乙酯部分活性最强（半数抑制浓度（IC50）：97μg/mL），其次是水相部分（IC50：109μg/mL）。关于脲酶抑制，正丁醇部分活性最强（IC50：97μg/mL）。然而，提取物对AChE和BChE未显示出显著抑制作用。在初步植物化学测试中，该植物地上部分显示含有皂苷、生物碱、黄酮类、酚类、单宁和萜类化合物。目前的研究结果可能归因于这些化合物组。
