Meilahti Clinical Proteomics Core Facility, Biochemistry and Developmental Biology, Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
Nephrol Dial Transplant. 2013 Jul;28(7):1648-56. doi: 10.1093/ndt/gft008. Epub 2013 Apr 3.
Matrix-assisted laser desorption ionization (MALDI)-profiling and imaging mass spectrometry are promising technologies for measuring hundreds of different molecules directly on tissues. For instance, small molecules, drugs and their metabolites, endogenous lipids, carbohydrates and complex peptides/proteins can be measured at the same time without significant disruption of sample integrity. In this review, the potential of MALDI-profiling/imaging technologies in disease proteomics, drug action and studies of cellular processes in the context of kidney tissue is described. Spatial and sequence information obtained in tissue MALDI-profiling/imaging studies can be correlated with other mass spectrometry-based techniques, auxiliary imaging technologies and routine (immuno) histochemical staining.
基质辅助激光解吸电离（MALDI）- profiling 和成像质谱分析是直接在组织上测量数百种不同分子的有前途的技术。例如，可以同时测量小分子、药物及其代谢物、内源性脂质、碳水化合物和复杂肽/蛋白质，而不会对样品完整性造成重大破坏。在这篇综述中，描述了 MALDI-profiling/imaging 技术在疾病蛋白质组学、药物作用以及肾脏组织中细胞过程研究中的潜力。在组织 MALDI-profiling/imaging 研究中获得的空间和序列信息可以与其他基于质谱的技术、辅助成像技术和常规（免疫）组织化学染色相关联。
