Proteomics Core Facility Biogenouest, IRSET, Campus de Beaulieu, Rennes Cedex, France.
Biol Reprod. 2012 Mar 19;86(3):74. doi: 10.1095/biolreprod.111.094896. Print 2012 Mar.
Matrix-assisted laser desorption/ionization (MALDI) tissue imaging mass spectrometry is particularly promising among the numerous applications of mass spectrometry. It is used for probing and analyzing the spatial arrangement of a wide range of molecules, including proteins, peptides, lipids, drugs, and metabolites, directly in thin slices of tissue. In the field of proteomics, the technology avoids tedious and time-consuming extraction and fractionation steps classically required for sample analysis. MALDI imaging mass spectrometry is increasingly recognized as a powerful method for clinical proteomics, particularly in cancer research. The technology has particular potential for the discovery of new tissue biomarker candidates, classification of tumors, early diagnosis or prognosis, elucidating pathogenesis pathways, and therapy monitoring. Over recent years, MALDI imaging mass spectrometry has been used for molecular profiling and imaging directly in male and female reproductive tissues. This review will consider some of the recent publications in the field, addressing a range of issues covering embryo development, gene expression product profiling during gametogenesis, and seeking and identifying biomarkers of reproductive cancers. The wealth of advances in mass spectrometry imaging will inevitably attract biologists and clinicians as the advantages and power of this technology become more widely known. This review will also discuss bottlenecks and the many technical issues that remain to be resolved before laboratories in the field can adopt the technology. We foresee that MALDI imaging mass spectrometry will have a major impact in reproductive research by opening new avenues to the understanding of various molecular mechanisms and the diagnosis of reproductive pathologies.
基质辅助激光解吸/电离（MALDI）组织成像质谱在质谱的众多应用中尤其有前景。它用于探测和分析广泛的分子（包括蛋白质、肽、脂质、药物和代谢物）在组织薄片中的空间排列。在蛋白质组学领域，该技术避免了经典的样品分析所需的繁琐和耗时的提取和分级步骤。MALDI 成像质谱技术越来越被认为是临床蛋白质组学的一种强大方法，特别是在癌症研究中。该技术在发现新的组织生物标志物候选物、肿瘤分类、早期诊断或预后、阐明发病机制途径以及治疗监测方面具有特殊潜力。近年来，MALDI 成像质谱已用于直接在男性和女性生殖组织中进行分子分析和成像。本文将考虑该领域的一些最新出版物，涵盖了一系列涵盖胚胎发育、配子发生过程中基因表达产物的分析、以及寻找和鉴定生殖癌的生物标志物等问题。质谱成像技术的大量进展不可避免地将吸引生物学家和临床医生，因为这项技术的优势和力量将越来越广为人知。本文还将讨论该领域实验室采用该技术之前仍需解决的瓶颈和许多技术问题。我们预计，MALDI 成像质谱将对生殖研究产生重大影响，为理解各种分子机制和生殖病理学的诊断开辟新途径。
