Dept. of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-900, São Paulo, SP, Brazil.
Biotechnol Prog. 2013 May-Jun;29(3):778-85. doi: 10.1002/btpr.1720. Epub 2013 Apr 1.
There is a worldwide interest in the development of processes for colorants production from natural sources such as microorganism. The aim of this study was to optimize red colorants production by Penicillium purpurogenum DPUA 1275 and to evaluate the effect of pH, temperature, salts and polymers on the stability of these colorants. Under optimized conditions, a 78% increase in red colorants production was achieved. The best pH and temperature conditions were obtained at pH 8.0 and 70°C, respectively. In the presence of salts NaCl and Na2 SO4 , both at concentrations of 0.1 and 0.5 M in Mcllvaine buffer (pH 8.0), the red colorants showed good stability. In the presence of both polymers polyethylene glycol and sodium polyacrylate, the red colorants kept their color intensity. Thus, this study presents characteristics of red colorants produced by P. purpurogenum that can be applied in different industries after toxicological examination.
从微生物等天然来源开发生产色素的工艺引起了全世界的关注。本研究旨在优化青霉(Penicillium purpurogenum)DPUA 1275 产生红色素的条件,并评估 pH 值、温度、盐和聚合物对这些色素稳定性的影响。在优化条件下,红色素的产量增加了 78%。最佳 pH 值和温度条件分别为 pH 8.0 和 70°C。在 NaCl 和 Na2 SO4 盐的存在下,它们在 McIlvaine 缓冲液(pH 8.0)中的浓度分别为 0.1 和 0.5 M,红色素表现出良好的稳定性。在聚乙二醇和聚丙烯酸钠两种聚合物的存在下,红色素保持了其颜色强度。因此,本研究介绍了青霉产生的红色素的特性,在经过毒理学检查后,可以应用于不同的行业。